AgeI-HF from New England Biolabs

Product Specs
ItemAgeI-HF
CompanyNew England Biolabs
Price $79.00 Inquire
Catalog NumberR3552S
Quantity300 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Dnmt3a-dependent de novo DNA methylation enforces lineage commitment and preserves functionality of memory Th1 and Tfh cellsCell ReportsMarch 24, 2026Bryant Perkins, Camille L. Novis, Andrew Baessler, Linda M. Sircy, Monyca M. Thomas, Malia Harrison-Chau, Andrew W. Richens, Bryce Fuchs, Nguyen X. Nguyen, Kaitlyn Flint, et al.Activator Dynabeads,Thermo Fisher Scientific,Catalog # 111456D AgeI-HF,New England Biolabs,Catalog #R3552S EcoRI,New England Biolabs,Catalog #R0101S Streptavadin-APC,Invitrogen,Catalog #S868 Influenza An

(2) DPEP2 suppresses hyperinflammation via metabolic reprogramming of macrophages in sepsisNature CommunicationsMarch 9, 2026Wenchen Luo, Wei Xu, Qimeng Yin, Jieqiong Song, Jie Wang, Yian Guan, Jian Huang, Jian Yu, Kefeng Zou, Danfeng Jin, et al.cooling to 4 °C. Next, 2 μg of the pLKO.1-puro plasmid77 (Addgene, 8453) was digested with 1 μL AgeI (R3552S, New England Biolabs, Ipswich, MA, USA), 1 μL EcoRI (R3101V, New England Biolabs), 2 μL CutSmart Buffer

(3) Integrating natural and engineered genetic variations to decode regulatory influence on blood traitsCell ReportsFebruary 24, 2026Manuel Tardaguila, Dominique Von Schiller, Michela Colombo, Ilaria Gori, Eve L. Coomber, Thomas Vanderstichele, Paola Benaglio, Chiara Chiereghin, Sebastian Gerety, Dragana Vuckovic, et al.beads,Beckman Coulter,Cat#A63881 AflII,New England Biolabs,Cat#R0520S AgeI-HF,New England Biolabs,Cat#R3552S T4 DNA ligase,New England Biolabs,Cat#M0202L NaeI,New England Biolabs,Cat#R0190S DpnI,New England Biolabs

(4) Systematic annotation of orphan RNAs reveals blood-accessible molecular barcodes of cancer identity and cancer-emergent oncogenic driversCell Reports MedicineFebruary 17, 2026Jeffrey Wang, Jung Min Suh, Brian J. Woo, Albertas Navickas, Kristle Garcia, Keyi Yin, Lisa Fish, Taylor Cavazos, Benjamin Hänisch, Daniel Markett, et al.intermediate pUC6 ligation product was then digested for 90 min using AgeI-HF (New England Biolabs Cat. #R3552S) and EcoRI-HF (New England Biolabs Cat. #R3101S). Digested inserts were then run on a 8% TBE gel, extracted,

(5) USP30-mediated Deubiquitination of Hexokinase 2 controls the metabolic fate of glucose and tumor progressionCell Death & DiseaseFebruary 14, 2026Zhang Haowei, Xiaolin Li, Weijie Liao, Xuan Qin, Yapei Jiang, Haitao Yang, Hongli Zeng, Yuetong Li, Weidong Xie, Yaou Zhang, et al.annealed shRNA oligos were cloned into the pLKO.1-TRC vector (Addgene, #10878) digested with AgeI (NEB, R3552S) and EcoRI (NEB, R3101S). The resulting plasmids were verified by restriction enzyme digestion and DNA sequencing

(6) Genetic landscape of human oocyte/embryo defectsCell GenomicsJanuary 14, 2026Biaobang Chen, Weijie Wang, Juanzi Shi, Xiaoxi Sun, Yichun Guan, Guimin Hao, Junli Zhao, Jian Mu, Zhihua Zhang, Fangzhou Xu, et al.10099-141C KOD-Plus-Mutagenesis Kit,TOYOBO,Cat# SMK-101 AgeI/ScaII restriction enzymes,New England BioLabs,Cat# R3552S HiScribe T7 ARCA mRNA Kit,New England BioLabs,Cat# E2065S RNeasy MinElute Cleanup Kit,Qiagen,Cat#

(7) A non-canonical ARMS-GABARAP interaction modulates dendritic spine formation and synaptic developmentThe EMBO JournalJanuary 8, 2026Wenli Jiang, Jin Ye, Jiasheng Chen, Xinyu Wang, Yahong Li, Jianchao Li, Yide Mei, Yanlu Lyu, Wei Hu, Chao Wangbuffer,Vazyme,P022-01 DNA marker,Vazyme,MD102-02 BamHI-HF,NEB,R3136L EcoRI-HF,NEB,R3101L Xhol,NEB,R0146L AgeI-HF,NEB,R3552S CutSmart,NEB,B7204S Proteinase K,MERCK,03115879001 Chloroquine,MCE,HY-17589A Trichloroacetic acid,Aladdin,T104257 Acetone,Sinopharm

(8) ALDH3A2 targets arachidonic acid to promote cell metastasis in TNBC via AMPK/m-TOR signaling pathwayBreast Cancer ResDecember 18, 2025Ding Wang, Shulei Luo, Rongji Yu, Doudou Wen, Hao Jiang, Keli Xu, Xiaotang Di, Shubing ZhangTable 1). Following sequence verification, the recombinant pLKO.1 vector was digested with AgeI (Cat#R3552S, NEB, USA) and EcoRI (Cat#R3101V, NEB, USA) for subsequent cloning steps. For ectopic expression constructs,

(9) Protocol for modeling injury-induced regeneration in retinal organoids and isolating hNRSCs for subretinal transplantation in rd10 miceSTAR ProtocolsDecember 1, 2025Ming Chen, YunXi Zhang, Na Gao, WenMin Pan, Chen Cheng, Xiaoyu Liu, ShaSha Li, JianZhong Su, Hui Liuprimary cell 4D-Nucleofector X Kit S,Lonza,Cat# V4XP-3032 T4PNK,NEB,Cat# M0201V AgeI-HF,NEB,Cat# R3552S BbsI-HF,NEB,Cat# R3539S EcoRI-HF,NEB,Cat# R3101V SalI-HF,NEB,Cat# R3138V HindIII-HF,NEB,Cat# R3104V MfeI-HF,NEB,Cat#

(10) Strategies to Screen and Evaluate Brain Targeting Antibodies Using an iPSC-Derived Blood–Brain Barrier ModelAntibodies (Basel)November 26, 2025Eun Seo Choi, Sophia Sahota, Emily Burnham, Yunfeng Ding, Eric V. Shustaof WT 46.1 insert in the pIRES-46.1-rabbitFc plasmid [24] using the NheI (NEB R3131S) and AgeI (NEB R3552S) restriction enzymes. For the histidine point mutants, gene blocks for each variant were codon optimized

(11) Growth Factor-Independent mTORC1 Signaling Promotes Primary Cilia Length via Suppression of AutophagyiScienceNovember 25, 2025Jong Shin, Khaled Tighanimine, Yann Cormerais, Dean M. Rosenthal, Pratima Niroula, Samuel C. Lapp, Krystle C. Kalafut, Madi Y. Cisse, Sandra Schrötter, Brendan D. ManningaattcaaaaaCAGGCCTATGGTCGAGATTTActcgagTAAATCTCGACCATAGGCCTG-3’ The Tet-pLKO-Puro plasmid was linearized by digestion with AgeI (NEB; R3552S) and EcoRI (NEB; R3101S) restriction enzymes to generate complementary overhangs for the annealed shMTOR

(12) Tumoral ALOX5 mediated arachidonic acid metabolism regulates immune response in non-small cell lung cancerCell Oncol (Dordr)November 5, 2025Yuan Gao, Yangchen Xia, Jiayao Li, Yongfeng Lai, Songlin Yin, Junhong Lin, Dongrunhan Yu, Shanshan Huang, Fujia Lu, Weimin Wang, et al.RNAs (shRNAs). Each shRNA oligonucleotide was cloned into the pLKO.1 vector digested with AgeI (NEB, R3552S) and EcoRI (NEB, R3101S), and subsequently confirmed by Sanger sequencing. Lentiviruses were then produced

(13) Quantifying the impact of genetic mutations on enhancer dynamicsbioRxivOctober 24, 2025Maitreya Das, Deepro Banerjee, Ayaan Hossain, Matthew Jensen, Saie Mogre, Jiawan Sun, Joseph Mao, Adam B. Glick, Howard M. Salis, Santhosh Girirajanrestriction enzymes SalI-HF (catalog # R3138S, New England Biolabs, MA, USA) and AgeI-HF (catalog #R3552S, New England Biolabs, MA, USA), as previously described22,24. We performed 24 independent digestion

(14) Enhancing cap-independent translation of linear mRNANat CommunOctober 16, 2025Sebastian Golojuch, Brendan Largey, Afaf H. El-Sagheer, Tom BrownNEBuilder® HiFi DNA assembly. The vector was obtained by digesting pmRNA-EGFP-A120 plasmid with AgeI-HF (#R3552S, New England Biolabs) and NotI-HF (#R3189S, New England Biolabs) restriction enzymes following the manufacturer’s

(15) Molecular profiling of adult C. elegans glia across sexes by single-nuclear RNA-seqDevelopmental CellOctober 6, 2025Maria D. Purice, Elgene J.A. Quitevis, R. Sean Manning, Liza J. Severs, Nina-Tuyen Tran, Violet Sorrentino, Connor Finkbeiner, Feinan Wu, Michael Zager, Manu Setty, et al.LS,Ambion,Cat#10296010 5Prime-Heavy Phase Lock Gel,VWR,Cat#10847-802 BamHI,NEB,Cat#R3136L SphI,NEB,Cat#R3182L AgeI,NEB,Cat#R3552S Alt-R S.p. Cas9 Nuclease V3,IDT,Cat#1081058 Alt-R CRISPR-Cas9 tracrRNA,IDT,Cat#1072532 Alt-R CRISPR-Cas9 crRNA

(16) Matrin-3 forms spherical and wormlike assemblies that are modulated by RNA binding and ALS/FTD-associated mutationsMolecular CellOctober 2, 2025Macy L. Sprunger, Min Kyung Shinn, Sabrina K. Talir, Ken Lee, Rohit V. Pappu, Meredith E. Jackrelrecombinant proteins","Chemicals, peptides, and recombinant proteins" AgeI-HF,New England Biolabs,Cat# R3552S BamHI-HF,New England Biolabs,Cat# R3136S DpnI,New England Biolabs,Cat# R0176S NotI-HF,New England Biolabs

(17) SARS-CoV-2 Nsp15 endoribonuclease subverts host defenses to enhance viral fitness in lung cellsJournal of VirologySeptember 23, 2025Xiang Chi, Xueying Liang, Kishore Vaddadi, Xiaoming Zhang, Chaitanya Gandikota, Sunil More, Lin Liu, Xufang Dengextracted using miniprep (Promega A1222). Diagnostic digestion with KpnI-HF (NEB R3142S) and AgeI (NEB R3552S) confirmed the correct constructs (expected size 621 bp), which were then sent for sequencing for further

(18) BRD2 Upregulation as a Pan-Cancer Adaptive Resistance Mechanism to BET InhibitionbioRxivSeptember 11, 2025Suyakarn Archasappawat, Juliette Jacques, EunJung Lee, Chang-il Hwangused. A total of 6 μg of plasmid DNA was digested with 1 μl EcoRI-HF (NEB, R3101T), 1 μl AgeI-HF (NEB, R3552S), and 5 μl rCutSmart Buffer (NEB, B6004S) in a 50 μl reaction and incubated at 37°C for 3 hours. To

(19) Pharmacological Targeting of DHHC9‐Mediated STRN4 Palmitoylation to Suppress YAP‐Driven Cancer MetastasisJournal of Cellular and Molecular MedicineSeptember 3, 2025Yang Tian, Wei Li, Qing Zhai, Ying Yu, Jiaxin Yuan, Yan Ma, Jingjing Yang, Mingyue Li, Wenwen Chang, Wenjing Li, et al.China",MP102‐02 Acetonitrile LC–MS grade,"Aladdin, China",A120771 AgeI‐HF,"New England Biolabs, U.S.",R3552S Ampicilin,"Beyotime, China",ST007‐5 g Anti‐DDDDK‐tag mAb,"MBL, Japan",M185 Anti‐HA‐tag mAb,"MBL,

(20) Escape from TGF‐β‐induced senescence promotes aggressive hallmarks in epithelial hepatocellular carcinoma cellsMolecular OncologySeptember 1, 2025Minenur Kalyoncu, Dilara Demirci, Sude Eris, Bengisu Dayanc, Ece Cakiroglu, Merve Basol, Merve Uysal, Gulcin Cakan‐Akdogan, Fang Liu, Mehmet Ozturk, et al.(TRCN0000219406) was established as previously described [20]. Briefly, hairpin oligonucleotides with AgeI (R3552S; NEB, Ipswich, MA, USA) and EcoRI (R3101S; NEB, Ipswich, MA, USA) restriction site overhangs were designed

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AgeI-HF

AgeI-HF from New England Biolabs

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New England Biolabs

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AgeI-HF from New England Biolabs

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