AgeI-HF from New England Biolabs

Product Specs
ItemAgeI-HF
CompanyNew England Biolabs
Price $325.00 Inquire
Catalog NumberR3552L
Quantity1500 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Technical and biological sources of noise confound multiplexed enhancer AAV screeningNat CommunApril 28, 2026Avery C. Hunker, John K. Mich, Naz Taskin, Amy Torkelson, Trangthanh Cardenas, Jean-Benoît Lalanne, Joseph T. Mahoney, Darren Bertagnolli, Anish Bhaswanth Chakka, Rushil Chakrabarty, et al.minimal promoters were digested overnight with 1 µL MluI-HF (NEB R3198L) and 1 µL and AgeI-HF (NEB R3552L) in 10× rCutsmart Buffer (NEB B6004S) diluted in water. The digested backbone (AiP11839) or inserts

(2) Single-cell Analysis Reveals Essential lncRNAs Regulating Human Trophoblast Lineage DifferentiationLife MedicineApril 17, 2026Weiyi Zhang, Chunyan Le, Shanru Yi, Wenqiang Liu, Xiaoyu Liu, Zhen Yin, Hong Wang, Rui Gao, Shaorong Gao, Jiayu Chenlentiviral vector system. The plasmid was linearized by double digestion with AgeI (New England Biolabs, R3552L)/EcoRI (New England Biolabs, R3101S) restriction endonuclease and purified for subsequent cloning. Using

(3) FGFR1/YAP1 signaling in endothelial cells drives renal fibrosis and offers a therapeutic targetFront PharmacolApril 13, 2026Xin Jiang, Yafeng Ren, Mengli Yan, Mingjing Li, Huixia Cao, Fengmin Shaowhich were then ligated into the pLKO.1 vector (Tsingke Biotechnology) using AgeI(New England Biolabs,#R3552L) and EcoRI(New England Biolabs,#R00101V) restriction sites. All plasmid constructs were validated by

(4) OSBPL3 promotes LUAD metastasis and glycolysis through transcriptional upregulation of PLAU via NFE2L2Translational OncologyApril 1, 2026Qingqiong Zhang, Jingshun Zhang, Junxian Li, Shuang Chen, Yijun Liu, Kaidi Li, Minzhang GuoBiotech (Shanghai). Restriction sites EcoRI (5′-GAATTC-3′; NEB #R0101L) and AgeI (5′-ACCGGT-3′; NEB #R3552L) were utilized for cloning. The annealed double-stranded oligonucleotides and PCR product were ligated

(5) Leveraging the germ layer development patterns to predict prognosis and identify MEST as a novel therapeutic target in gliomaCancer Cell InternationalJanuary 7, 2026Wei Zhang, Shunjin Xia, Yanming Xiao, Hongwei Liu, Chaoqian Wang, Luohuan Dai, Yinhua Chen, Xuelei Lin, Hongyi Liu, Nian Jianglentiviral package The PLKO.1 vector was digested with EcoRI and AgeI restriction enzymes (#R310S,#R3552L, NEB, USA) and subsequently ligated to a short hairpin structure using T4 DNA ligase (#M0202S, NEB,

(6) The NLRP6 inflammasome is activated by sterile or pathogen-induced endolysosomal damageThe EMBO JournalNovember 20, 2025Alexandra Boegli, Elliott M Bernard, Louise Lacante, Gaël Majeux, Ella Hartenian, Vanessa Mack, Petr Broz#R045A) and was cloned into pLJM1_EGFP (addgene #19319) backbone, using AgeI-BstBI (New England Biolabs #R3552L and #R0519L) restriction digest and In-Fusion cloning (Takara Bio #6396650). In this pLJM1 backbone,

(7) One-pot cloning and protein expression platform for genetic engineeringbioRxivSeptember 11, 2025Wakana Sato, Judee Sharon, Brock Cash, Christopher Deich, Nathaniel J. Gaut, Joseph Heili, Aaron E. Engelhart, Katarzyna P. Adamala(M0492S, NEB). After PCR, the amplified genes were agarose gel purified and digested with with AgeI-HF (R3552L, NEB) and MluI-HF (R3198S, NEB) in 50 μl reaction containing 1x CutSmart® Buffer (NEB, B7204S). After

(8) SOD2 is a regulator of proteasomal degradation promoting an adaptive cellular starvation responseCell ReportsApril 22, 2025Nurul Khalida Ibrahim, Sabine Schreek, Buesra Cinar, Anna Sophie Stasche, Su Hyun Lee, Andre Zeug, Tim Dolgner, Julia Niessen, Evgeni Ponimaskin, Halyna Shcherbata, et al.,Thermo Fisher Scientific,Cat#11875119 OptiMEM,Thermo Fisher Scientific,Cat#31985062 Polyethyleneimine,Sigma-Aldrich,Cat#408727 AgeI-HF,NEB,Cat#R3552L MluI-HF,NEB,Cat#R3198S BamHI-HF,NEB,Cat#R3136S EcoRI-HF,NEB,Cat#R3101S Sucrose,Sigma-Aldrich,Cat#S0389 Horseradish

(9) Systemic genome-epigenome analysis captures a lineage-specific super-enhancer for MYB in gastrointestinal adenocarcinomaMolecular Systems BiologyApril 15, 2025Fuyuan Li, Shangzi Wang, Lian Chen, Ning Jiang, Xingdong Chen, Jin Li,New England Biolabs,N0446S NEBuffer™ 2,New England Biolabs,B7002S EcoRI-HF®,New England Biolabs,R3101L AgeI-HF®,New England Biolabs,R3552L 0.5 M DTT,Beyotime,ST041-10ml Dual Luciferase Reporter Gene Assay Kit,Beyotime,RG027 Cell Counting

(10) Dynamic interaction of MYC enhancer RNA with YEATS2 protein regulates MYC gene transcription in pancreatic cancerEMBO ReportsApril 11, 2025Jayita Roy, Aniket Kumar, Shouvik Chakravarty, Nidhan K Biswas, Srikanta Goswami, Anup Mazumder,New England Biolabs,E0552S Lipofectamine 3000,Invitrogen,L3000015 NheI,NEB,R313S EcoRI,NEB,R3101S XbaI,NEB,R0145S AgeI,NEB,R3552L NcoI,NEB,R3193S Software,Software,Software UCSC genome browser,,https://genome.ucsc.edu/ Adobe Photoshop

(11) Nanobody fusion enhances production of difficult-to-produce secretory proteinsJournal of Biological ChemistryFebruary 12, 2025Runchuan Yan, Yan Zhang, Hui Zhang, Jiyan Mawhich was provided by the CIBR Vector Core. These vectors were prepared by digesting with Age I (cat# R3552L, NEB) and Eco RI (cat# R0101S, NEB) enzymes, followed by ligation of annealed shRNA primers using T4 DNA ligase

(12) Divergent Effects of Circoviridae Capsid Proteins on Type I Interferon SignalingPathogensJanuary 13, 2025Anon H. Kosaka, Chen-Yu Huang, Zih-Ying Lu, Hua-Zhen Hsing, Amonrat Choonnasard, Rissar Siringo Ringo, Kuo Pin Chuang, Akatsuki Saitoplasmid [21], which was prelinearized with AgeI–HF (New England Biolabs (NEB), Ipswich, MA, USA, Cat# R3552L) and NheI–HF (NEB, Cat# R3131L) using an In-Fusion HD Cloning Kit (TaKaRa, Kusatsu, Japan, Cat# Z9633N).

(13) qTAG: an adaptable plasmid scaffold for CRISPR-based endogenous taggingThe EMBO JournalDecember 12, 2024Reuben Philip, Amit Sharma, Laura Matellan, Anna C Erpf, Wen-Hsin Hsu, Johnny M Tkach, Haley D M Wyatt, Laurence PelletierMedium,Gibco,31985070 Puromycin,InvivoGen,ant-pr-1 Blasticidin,InvivoGen,ant-bl-1 Zeocin,InvivoGen,ant-zn-1 AgeI-HF,New England Biolabs,R3552L BamHI-HF,New England Biolabs,R3136S EcoRI-HF,New England Biolabs,R3101S HindIII-HF,New England Biolabs,R3104S KpnI-HF,New England Biolabs

(14) Identification of suitable target/E3 ligase pairs for PROTAC development using a rapamycin-induced proximity assay (RiPA)eLifeDecember 6, 2024Bikash Adhikari, Katharina Schneider, Mathias Diebold, Christoph Sotriffer, Elmar WolfPolymerase,Thermo Fisher Scientific,Cat# F630L, "Peptide, recombinant protein",AgeI-HF,New England BioLabs,Cat# R3552L, "Peptide, recombinant protein",AscI,New England BioLabs,Cat# R0558L, "Peptide, recombinant protein",BamHI-HF,New England BioLabs

(15) Dysregulation of the 3β-hydroxysteroid dehydrogenase type 2 enzyme and steroid hormone biosynthesis in chronic kidney diseaseFront Endocrinol (Lausanne)October 25, 2024Yiyi Zuo, Dongqing Zha, Yue Zhang, Wan Yang, Jie Jiang, Kangning Wang, Runze Zhang, Ziyi Chen, Qing He(ENST00000369416; 201-exon4) into PLKO.1 vector. The circular PLKO.1 plasmid was digested by AgeI (R3552L, NEB, Ipswich, MA, USA) and EcoRI (R3101L, NEB, Ipswich, MA, USA), and the prepared open vector was

(16) Drupacine as a potent SARS-CoV-2 replication inhibitor in vitroBiosafety and HealthOctober 1, 2024Chen Yang, Yanying Yu, Qi Peng, Jingwei Song, Bo Sun, Yi Shi, Qiang Dingplasmid bearing the SARS-CoV-2 sGluc replicon cDNA was linearized using AgeI-HF restriction endonuclease (R3552L, NEB). The linearized plasmid served as the template for in vitro transcription using the mMESSAGE mMACHINE T7 Transcription Kit

(17) Streamlined process for effective and strand-selective mitochondrial base editing using mitoBEsBiophysics ReportsAugust 31, 2024Xiaoxue Zhang, Zongyi Yi, Wei Tang, Wensheng Weino. R3131M) • SpeI-HF (New England Biolabs, cat. no. R3133M) • AgeI-HF (New England Biolabs, cat. no. R3552L) • PmeI (New England Biolabs, cat. no. R0560L) • HiScribe® T7 High Yield RNA Synthesis Kit (New England Biolabs

(18) The interaction between RIPK1 and FADD controls perinatal lethality and inflammationCell ReportsJune 25, 2024Diego A. Rodriguez, Bart Tummers, Jeremy J.P. Shaw, Giovanni Quarato, Ricardo Weinlich, James Cripps, Patrick Fitzgerald, Laura J. Janke, Stephane Pelletier, Jeremy Chase Crawford, et al.tlrl-pic Recombinant Murine TNF,Prepotech,Cat# 315-01A Restriction enzyme AgeI,New England BioLabs,Cat# R3552L Restriction enzyme HindIII,New England BioLabs,Cat# R0104L RIPK3 inhibitor (GSK’872),Invivogen,Cat#

(19) New AAV9 engineered variants with enhanced neurotropism and reduced liver off-targeting in mice and marmosetsiScienceMay 17, 2024Serena Gea Giannelli, Mirko Luoni, Angelo Iannielli, Jinte Middeldorp, Ingrid Philippens, Simone Bido, Jakob Körbelin, Vania Broccolienzyme,NEB,Cat# R0569L AfeI enzyme,NEB,Cat# R0652L NheI-HF enzyme,NEB,Cat# R3131L AgeI-HF enzyme,NEB,Cat# R3552L Luria Broth (LB),MERCK,Cat# L3022-1kg Ampicillin,MERCK,Cat# A9518 PolyFect Transfection Reagent,Qiagen,Cat#

(20) FAM111B Acts as an Oncogene in Bladder CancerCancers (Basel)October 24, 2023Ning Huang, Lei Peng, Jiaping Yang, Jinqian Li, Sheng Zhang, Mingjuan SunCo., Ltd., Shanghai, China) were prepared by restriction enzymes AgeI (NEB, Ipswich, MA, USA, Cat. R3552L) and EcoRI (NEB, USA, Cat. R3101L). These two sequences were ligated via T4 DNA ligase, and the recombinant

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AgeI-HF

AgeI-HF from New England Biolabs

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New England Biolabs

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AgeI-HF from New England Biolabs

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