AflIII from New England Biolabs

Product Specs
ItemAflIII
CompanyNew England Biolabs
Price $75.00 Inquire
Catalog NumberR0541S
Quantity250 units
Concentration5 u/µL
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

A/C(AG)(CT)GT

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Highly efficient expression of DNA-peptide conjugates in growth-arrested cellsNature CommunicationsJanuary 7, 2026Zulfiqar Y. Mohamedshah, Chih-Chin Chi, Ember M. Tota, Alexis C. Komor, Neal K. Devaraj(Supplementary Method 1). For ABE8e-P2A-eGFP gene cassettes, a similar strategy was applied using AflIII (NEB, R0541S) instead of AvrII, with an AflIII sticky-end DNA oligonucleotide for ligation (Supplementary Method

(2) Pathways and products of base excision DNA repair in Xenopus laevis eggs: contrast with human cell pathwaysNucleic Acids ResDecember 10, 2025Zhouyiyuan Xue, Patrick James Sutton, John D Haley, Christopher W Brownlee, Bruce Dempleeliminate an extra Nt.BspQI site located outside the N3 region, HindIII (NEB, R0104S) and AflIII (NEB, R0541S) enzymes were employed. Using 30 units each of HindIII and AflIII, 10 μg of previously purified plasmid

(3) Comprehensive profiling of activity and specificity of RNA-guided transposons reveals opportunities to engineer improved variantsNucleic Acids ResearchSeptember 23, 2025Seong Guk Park, Jung-Un Park, Esteban Dodero-Rojas, John A Bryant, Geetha Sankaranarayanan, Elizabeth H KelloggM0202L) in a one-step Golden Gate assembly. The barcoded TnsB library was digested with AflIII (NEB, R0541L) and sent to the Hartwell Center in St. Jude Children’s Research Hospital for Revio PacBio sequencing

(4) Protocol to screen chemicals to enhance homology-directed repair in CRISPR-Cas9 gene editingSTAR ProtocolsSeptember 19, 2025Min Ju Jo, Hye Jin NamDimethyl sulfoxide (DMSO),Sigma-Aldrich,Cat#D8418 KpnI-HF,New England Biolabs,Cat#R3142L AflIII,New England Biolabs,Cat#R0541S AatII,New England Biolabs,Cat#R0117S BbsI,New England Biolabs,Cat#R0539S T4 DNA ligase,New England Biolabs

(5) Human genetic variation reveals FCRL3 is a lymphocyte receptor for Yersinia pestisCell GenomicsSeptember 10, 2025Rachel M. Keener, Sam Shi, Trisha Dalapati, Liuyang Wang, Nicolás M. Reinoso-Vizcaino, Micah A. Luftig, Samuel I. Miller, Timothy J. Wilson, Dennis C. KoMedium,ThermoFisher,Cat#00-4958-02 mini-cOmplete protease inhibitor tablet,Sigma,Cat#11836153001 AflIII,NEB,Cat#R0541 HINDIII-HF,NEB,Cat#R3104 AsiSI,NEB,Cat#R0630 XbaI,NEB,Cat#R0145 ,, Critical commercial assays,Critical

(6) Sm-site containing mRNAs can accept Sm-rings and are downregulated in Spinal Muscular AtrophyNucleic acids researchAugust 11, 2025Blatnik AJ 3rd, Sanjeev M, Slivka J, Pastore B, Embree CM, Tang W, Singh G, Burghes AHM.PmlI (New England Biolabs R0532L), AfeI (New England Biolabs R0652L), or AflIII (New England Biolabs R0541L). Long RNAs were in vitro transcribed using the HiScribe T7 High Yield Kit (New England Biolabs SE20405).

(7) Sequence and trajectory of early Alzheimer's disease-related tau inclusions in the hippocampal formation of cases without amyloid-β depositsActa NeuropatholMay 23, 2025Heiko Braak, Benjamin Mayer, Simone Feldengut, Michael Schön, Kelly Del TrediciAliquots of the PCR-amplified products were digested with restriction enzyme AlfIII (New England BioLabs R0541S, Ipswich, MA; 10U/µl), HaeII (New England BioLabs R0107S; 20U/µl), and HaeI (New England BioLabs R0139S;

(8) Intercellular bridges are essential for transposon repression and meiosis in the male germlineNat CommunFebruary 10, 2025Julia Sorkin, Kevin Tilton, Matthew A. Lawlor, Shreya N. Sarathy, Shun Liang, Angelina Albanese, Mashiat Rabbani, Saher S. Hammoud, Christopher E. Ellison, Florencia Pratto, et al.(5′-GCCAAAGGAACTGAAAGAGAAAAATAC) primers, followed by digestion of the amplified product with AflIII restriction enzyme (NEB R0541S). The tama mutation creates an AflIII restriction site; AflIII cuts the mutant allele (184 bp and 26

(9) Ciliary Proteins Repurposed by the Synaptic Ribbon: Trafficking Myristoylated Proteins at Rod Photoreceptor SynapsesInternational journal of molecular sciencesJune 27, 2022Suiwal S, Dembla M, Schwarz K, Katiyar R, Jung M, Carius Y, Maxeiner S, Lauterbach MA, Lancaster CRD, Schmitz F.restriction analysis of plasmid DNA for the silent restriction site AflIII (NEB, Frankfurt, Germany, R0541S) present in the point-mutated sequences. Positive clones were further verified by Sanger sequencing. Cloning

(10) Germinal Center B Cells Replace Their Antigen Receptors in Dark Zones and Fail Light Zone Entry when Immunoglobulin Gene Mutations are DamagingImmunitySeptember 18, 2018Stewart I, Radtke D, Phillips B, McGowan SJ, Bannard O.214-14 Q5 High Fidelity DNA polymerase,New England Biolabs,Cat# M0491L AFlIII,New England Biolabs,Cat# R0541S NsiI-HF,New England Biolabs,Cat# R3127S ,, Critical commercial assays,Critical commercial assays,Critical

(11) Designing and overproducing a tandem epitope of gp350/220 that shows a potential to become an EBV vaccineHeliyonMarch 1, 2018Widodo, Veronica Margarecaesha Anyndita N, Dluha N, Rifa'i M, Himmah K, Wahyuningsih MD.results were checked for validity based on the excision of the restriction enzyme (AflIII; NEB Cat No. R0541S). Following, the E. coli strain BL21 was transformed using a newly constructed plasmid. Briefly, the

(12) Extraction of amplifiable DNA from embalmed human cadaver tissueBMC Research NotesDecember 13, 2017Lindsay Gielda, Stefanie Riggpolymerase (Promega cat#M7132). Amplified DNA was digested with HaeII and AflIII (NEB cat#R0107S and R0541S) for 2 h at 37 °C and resolved on a 4% agarose gel. Samples were classified based on size; 145, 168,

(13) Targeting a Single Alternative Polyadenylation Site Coordinately Blocks Expression of Androgen Receptor mRNA Splice Variants in Prostate CancerCancer researchOctober 1, 2017Van Etten JL, Nyquist M, Li Y, Yang R, Ho Y, Johnson R, Ondigi O, Voytas DF, Henzler C, Dehm SM.with Qiaquick PCR purification kit (28104 Qiagen, Valencia, CA) and digested with 10 units of Afl III (R0541S New England Biolabs, Ipswich, MA) for 2 hours. Restriction analysis was performed via agarose gel electrophoresis. siRNA

(14) Light-Dependent Regulation of Sleep and Wake States by Prokineticin 2 in ZebrafishNeuronJuly 5, 2017Shijia Chen, Sabine Reichert, Chanpreet Singh, Grigorios Oikonomou, Jason Rihel, David A. Prober5′-GGCGTTGGTAATTGCGTATT-3′ and 5′-TATCAGCCACCAGCACTCTG-3′. PCR products were digested with AflIII (R0541, New England Biolabs Inc.). Mutant (527 bp) and WT (218 bp + 316 bp) bands were distinguished by running

(15) Erratum to: ‘The Memory Aid study: protocol for a randomized controlled clinical trial evaluating the effect of computer-based working memory training in elderly patients with mild cognitive impairment (MCI)’TrialsDecember 1, 2016Marianne M. Flak, Susanne S. Hernes, Linda Chang, Thomas Ernst, Vanessa Douet, Jon Skranes, Gro C. C. LøhaugenMA) for 2 hours at 37 °C, and by HaeII (R0107S, New England Biolabs, Beverly, MA, USA) and AflIII (R0541S, New England Biolabs, Beverly, MA, USA) overnight at 37 °C. The digested PCR products will then be analyzed

(16) IMAGEtags: Quantifying mRNA Transcription in Real Time with Multiaptamer ReportersVisualizing RNA Dynamics in the CellJune 1, 2016Judhajeet Ray, Ilchung Shin, Muslum Ilgu, Lee Bendickson, Vinayak Gupta, George A. Kraus, Marit Nilsen-Hamiltonsynthetic oligonucleotides Digest pZErO-2 vector with AflIII (New England Biolabs-NEB, Ipswich, MA, Cat# R0541S) and ApoI (NEB, R0566S) restriction enzymes and isolate the 1854 base pair fragment using gel electrophoresis

(17) Amplification refractory mutation system polymerase chain reaction versus optimized polymerase chain reaction restriction-fragment length polymorphism for apolipoprotein E genotyping of majorly depressed patientsMolecular Medicine ReportsNovember 1, 2015HONGMIN YOU et al.72˚C for 15 min. The AflIII digestion mixture contained 10 µl PCR products and five units of AflIII (R0541L; New England Biolabs, Ipswich, WI, USA) in the buffer supplied by the manufacturer (NEB 3 buffer). Similarly,

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AflIII

AflIII from New England Biolabs

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AflIII from New England Biolabs

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