Phosphatase Inhibitor Cocktails are a mix of chemicals that act as inhibitors of phosphatase enzymes. Phosphatases in crude extracts may hydrolyze and dephosphorylate compounds of biological importance and, therefore, inhibitor cocktails are used to help prevent this degradation. These reagents help to improve the yield of specific, native proteins from the sample. Inhibitors specific to a particular phosphatase can be found, as can broad-spectrum inhibitor cocktails. Broad-spectrum inhibitor cocktails can inhibit acidic and alkaline phosphatases, serine/threonine phosphatases, and protein tyrosine phosphatases (PTPs). The cocktails contain chemicals such as sodium fluoride, sodium pyrophosphate, ß-glycerophosphate, sodium orthovanadate, and sodium tartrate dihydrate in various combinations. Phosphatase inhibitor cocktails can be used during cell lysis, for the study of proteins and their purification and isolation, prior to Western Blotting, and during the study of cellular pathways. Visit the supplier page for more product information.
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