TEV Protease

Tobacco Etch Virus (TEV) protease is a cysteine protease enzyme with a molecular weight of 27 kDa. It is highly sequence-specific, with a recognition sequence of Glu-Asn-Leu-Tyr-Phe-Gln?Gly/Ser. Cleavage occurs between the Gln and Gly/Ser residues. The enzyme is active even at low temperatures and its high specificity makes it ideal for use in molecular biology, in particular, for cleaving fusion tags from recombinant proteins. Because the plant-based target sequence also rarely occurs in animals, the protease is deemed relatively non-toxic. Modified variants of TEV protease have been developed, such as AcTEV and TurboTEV, which feature greater stability and specificity. TEV protease containing fusion tags are also available for easy removal and cleavage. Visit the enzyme supplier page for more product information.