Leica EM Cryo-CLEM from Leica Microsystems

Product Specs
ItemLeica EM Cryo-CLEM
CompanyLeica Microsystems
Price Get Pricing Get Quote for this Product
Catalog NumberCRYO-CLEM
QuantityEA
TypeCorrelative Light and Electron Microscope
ApplicationsSample transfer and loading; transfer of coordinates to different EM software solutions

Specifications/Features

The cryo objective with low working distance (0.28 mm) and with NA 0.9 for high resolution (364 nm) ensures fast and specific localization of target structures in EM

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Leica Microsystems

10 Parkway North Blvd., Suite 300

Deerfield, Illinois 60015

United States

Phone: 800 248 0123

Company Profile

Website: www.leica-microsystems.com

(1) Ebola virus inclusion bodies are liquid organelles whose formation is facilitated by nucleoprotein oligomerizationEmerging Microbes & InfectionsDecember 8, 2023Bianca S. Bodmer, Melina Vallbracht, Dmitry S. Ushakov, Lisa Wendt, Petr Chlanda, Thomas Hoenenand vitrified using a Leica EM GP2 automatic plunge-freezer. The infected cells were imaged using a Cryo-CLEM wide-field fluorescent microscope (Leica Microsystems). Z-stacks were acquired with a z-size of 30 μm

(2) Correlative montage parallel array cryo-tomography for in situ structural cell biologyNature MethodsOctober 1, 2023Jie E. Yang, Matthew R. Larson, Bryan S. Sibert, Joseph Y. Kim, Daniel Parrell, Juan C. Sanchez, Victoria Pappas, Anil Kumar, Kai Cai, Keith Thompson, et al.systems45–47. To further confirm the preservation of an ROI in the lamella, lamellae were transferred to the Leica EM Cryo-CLEM THUNDER28 or Leica Stellaris cryo-confocal system and a z-stack of the same channels was collected.

(3) Effects of exercise training on neurological recovery, TGF-β1, HIF-1α, and Nogo-NgR signaling pathways after spinal cord injury in ratsClinics (Sao Paulo)July 27, 2023Xubin Ji, Zhaowan Xu, Dayong Liu, Yangwang Chenlongitudinally to 20 μm thickness and stained with HE staining. Morphological changes were observed under EM Cryo CLEM-type light microscopy (Leica Microsystems [Shanghai] Trading Co., Ltd., China), and 10 fields were randomly

(4) Quantifying organellar ultrastructure in cryo-electron tomography using a surface morphometrics pipelineJournal of Cell BiologyApril 3, 2023Benjamin A. Barad, Michaela Medina, Daniel Fuentes, R. Luke Wiseman, Danielle A. Grotjahntiled image maps (atlases) of EM grids containing vitrified cellular samples were acquired with a Leica CryoCLEM microscope (Leica) and collected using Leica LAS X software (25 um Z stacks with system optimized steps,

(5) The architecture of Cidec-mediated interfaces between lipid dropletsCell ReportsFebruary 16, 2023Iva Ganeva, Koini Lim, Jerome Boulanger, Patrick C. Hoffmann, Olivia Muriel, Alicia C. Borgeaud, Wim J.H. Hagen, David B. Savage, Wanda Kukulskiwere screened at −195°C for ice quality and for regions featuring cells of interest by cryo-FM in a Leica EM cryo-CLEM system (Leica Microsystems) in a humidity-controlled room. The system used to image Cidec-EGFP and untagged

(6) The Use of Panitumumab-IRDye800CW in a Novel Murine Model for Conjunctival Squamous Cell CarcinomaTranslational Vision Science & TechnologyJuly 27, 2022Gun Min Youn et al.with Cytoseal 60 (Thermo Fisher) on coverslips. Imaging was performed using Leica Thunder Imager EM Cryo CLEM Microscope (Leica Biosystems) at 2.5X, 10X and 20X magnification. Statistical Analysis Wilcoxon signed-rank

(7) Reconstitution of kinetochore motility and microtubule dynamics reveals a role for a kinesin-8 in establishing end-on attachmentseLifeJuly 5, 2022Julia R Torvi, Jonathan Wong, Daniel Serwas, Amir Moayed, David G Drubin, Georjana Barnesnitrogen until they were imaged. Cryo-fluorescence light microscopy Cryo-samples were imaged on a Leica EM Cryo CLEM system (Leica Microsystems). The system consists of a Leica DM6 FS widefield microscope that is equipped

(8) Bimodal endocytic probe for three-dimensional correlative light and electron microscopyCell Reports MethodsMay 23, 2022Job Fermie et al.II (Thermo Fisher) kept at 37°C, 100% humidity. Cryo fluorescence microscopy was performed using a Leica EM cryo-CLEM wide-field microscope (Leica Micosystems) equipped with a 50x/0.90 NA DRY cryo-objective lens. 15 μm

(9) Sample preparation and image registration for correlative cryo-FM and cryo-FIB-SEM of plunge-frozen mammalian cellsSTAR ProtocolsMarch 18, 2022Nadav Scher, Katya Rechav, Perrine Paul-Gilloteaux, Ori Avinoamliquid N2 storage system until imaging. Cryo-fluorescence microscopy Timing: 2–3 h per grid Leica cryo-CLEM FM transfer shuttle can accommodate clipped grids. For older cryo-CLEM models and workflow, including

(10) PNP Hydrogel Prevents Formation of Symblephara in Mice After Ocular Alkali InjuryTranslational Vision Science & TechnologyFebruary 22, 2022Aditi Swarup, Abigail K. Grosskopf, Lindsay M. Stapleton, Varun R. Subramaniam, BaoXiang Li, Irving L. Weissman, Eric A. Appel, Albert Y. Wu4′,6-diamidino-2-phenylindole (DAPI) for 5 minutes. Fluorescent images were taken using the THUNDER Imager EM Cryo CLEM microscope (Leica Microsystems, Wetzlar, Germany) at 10× and 20× magnifications. For Masson's trichrome

(11) Practical Approaches for Cryo-FIB Milling and Applications for Cellular Cryo-Electron TomographyMethods Mol BiolFebruary 3, 2022Vinson Lam, Elizabeth Villaacquisition. There are commercially available cryo-fluorescence microscopes such as the Corrsight and the Leica Cryo-CLEM system. Additionally, aftermarket cryo-stage additions such as CMS-196 Linkam stage are available in

(12) A modular platform for automated cryo-FIB workflowseLifeDecember 24, 2021Sven Klumpe et al.cryo-glue (2:3 ethanol:isopropanol mixture) for cryo-confocal imaging with a Leica TCS SP8 equipped with Leica EM cryo-CLEM stage. Imaging was performed using a HC PL APO ×50/0.9 DRY objective in fluorescence mode using a 552

(13) Post-correlation on-lamella cryo-CLEM reveals the membrane architecture of lamellar bodiesCommunications BiologyDecember 1, 2021Steffen Klein, Benedikt H. Wimmer, Sophie L. Winter, Androniki Kolovou, Vibor Laketa, Petr Chlandasample transfer. In this study, we implemented an alternative strategy and developed a robust on-lamella cryo-CLEM approach, taking advantage of post-correlation. It is suitable for adherent cells cultured directly

(14) Electron cryo-tomography reveals the subcellular architecture of growing axons in human brain organoidseLifeOctober 26, 2021Hoffmann PC, Giandomenico SL, Ganeva I, Wozny MR, Sutcliffe M, Lancaster MA, Kukulski W.grids were screened for ice thickness and fluorescent signals within axon tracts by cryo-FM with the Leica EM cryo-CLEM system. The system was equipped with a HCX PL APO 50 x (0.9 NA) cryo-objective (Leica Microsystems),

(15) In situ fiducial markers for 3D correlative cryo-fluorescence and FIB-SEM imagingiScienceJuly 23, 2021Nadav Scher, Katya Rechav, Perrine Paul-Gilloteaux, Ori Avinoamrings (Thermo Fisher Scientific) under liquid nitrogen prior to imaging. Imaging was performed using a cryo-CLEM microscope (Leica Microsystems) as previously described (Schorb et al., 2017). Samples were mounted

(16) A phase-separated nuclear GBPL circuit controls immunity in plantsNatureJune 1, 2021Huang S, Zhu S, Kumar P, MacMicking JD.within the O-ring and C-ring (ThermoFisher Scientific, cryo-FIB autogrid and C-clip, respectively). Cryo-CLEM (Leica Microsystems EM Cryo-CLEM microscope) was performed as previously described54. Grids were transferred

(17) CorRelator: Interactive software for real-time high precision cryo-correlative light and electron microscopyJournal of Structural BiologyJune 1, 2021Jie E. Yang, Matthew R. Larson, Bryan S. Sibert, Samantha Shrum, Elizabeth R. Wrighttested on a Leica DMi8 widefield fluorescence microscope under ambient environmental conditions or a Leica EM Cryo-CLEM microscope system under cryo-conditions (Leica MicroSystems, Germany). For room-temperature correlation,

(18) Setting up and operating a cryo-EM laboratoryQuarterly Reviews of BiophysicsJanuary 8, 2021Deryck J. Millshttps://doi.org/10.1017/S003358352000013X 6 Deryck J. Mills Fig. 3. Room for CLEM. (a) Aquilos cryo-FIB-SEM (TFS) with Leica SP8 confocal cryo-CLEM in the background. (b) Close-up of the cryo-CLEM. (c) Front view of the Aquilos FIB-SEM. are the Chameleon

(19) In situ cryo-electron tomography reveals filamentous actin within the microtubule lumenJournal of Cell BiologySeptember 7, 2020Danielle M. Paul, Judith Mantell, Ufuk Borucu, Jennifer Coombs, Katherine J. Surridge, John M. Squire, Paul Verkade, Mark P. Doddingthese were plunge-frozen in liquid ethane using a Leica EM GP plunge freezer and transferred to a Leica CryoCLEM stage based on the Leica DM6000FS fluorescence light microscope. In this microscope, the objective is

(20) Preparing samples from whole cells using focused-ion-beam milling for cryo-electron tomographyNature ProtocolsJune 1, 2020Felix Roman Wagner, Reika Watanabe, Ruud Schampers, Digvijay Singh, Hans Persoon, Miroslava Schaffer, Peter Fruhstorfer, Jürgen Plitzko, Elizabeth Villascrewdriver (to close/open the shuttle clamp) Cryo-fluorescence microscope, e.g., CorrSight (FEI) or cryo CLEM (Leica) Cryo-transmission electron microscope where AutoGrids can be loaded, e.g., Titan Krios or

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Leica EM Cryo-CLEM from Leica Microsystems

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Leica EM Cryo-CLEM from Leica Microsystems

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