Agilent BioTek Lionheart LX Automated Microscope from Agilent Technologies

Product Specs
ItemAgilent BioTek Lionheart LX Automated Microscope
CompanyAgilent Technologies
Price Get Pricing Get Quote for this Product
Catalog NumberLionheart LX
TypeAutomated Microscrope
ApplicationsCell counting, Endpoint live cell assays (including apoptosis, autophagy, cytotoxicity and others), Cell cycle, Mitochondrial membrane potential, Fluorescence in-situ hybridization, Histology (H&E), Label-free cell counting, Confluence, Genotoxicity (including comet assay, gH2AX, immunofluorescence, NASH assays using lipophilic fluorescent probes, and phenotypic assays)
SoftwareGen5 Microplate Reader and Imager Software included
Plate Formats6- to 1536-well plates, Microscope slides, Petri and cell culture dishes, cell culture flasks (T25, T75), counting chambers (hemocytometers), chamber slides. Support for labware up to 1.5" tall
ModeFluorescence, high contrast brightfield and color brightfield
MethodSingle color, multi-color, montage, time lapse, Z-stack, z-stack montage, burst mode
Light SourceHigh power LEDs
Camera16-bit CMOS camera. 2.3 MP Sony IMX249 1/1.2" sensor with 5.86 micron pixel size
Dimensions(WxDxH) 17.9 x 18.3 x 14.1 inches (or 45.5 x 46.5 cm x 35.8 cm)
Regulatory StatusCE and TUV marked. RoHS compliant. Models for In Vitro Diagnostic use are available
QuantityEA

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Agilent Technologies

5301 Stevens Creek Blvd

Santa Clara, California 95051

United States

Phone: +1 877-424-4536

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Website: www.agilent.com

(1) pH-sensing G protein-coupled orphan receptor GPR68 is expressed in human cartilage and correlates with degradation of extracellular matrix during OA progressionPeerJDecember 5, 2023Nazir M. Khan, Martha E. Diaz-Hernandez, William N. Martin, Bhakti Patel, Samir Chihab, Hicham Drissipreviously (Khan et al., 2017b; Khan et al. 2023b). The cartilage sections were imaged using BioTek Lionheart LX Automated Microscope as described previously (Khan et al., 2020a, 2023a). Animals and induction of

(2) Towards a comprehensive regulatory map of Mammalian GenomesResearch squareSeptember 28, 2023Gonçalves TM, Stewart CL, Baxley SD, Xu J, Li D, Gabel HW, Wang T, Avraham O, Zhao G.(Thermo Fisher #A-21245). Images were taken under a microscope (SMZ-745T Zoom Stereo Photo Microscope, Nikon and Lionheart LX automated microscope, BioTek) and images were analyzed with Nikon NIS-Elements and Gen5 software. DNA The

(3) Differential chondrogenic differentiation between iPSC derived from healthy and OA cartilage is associated with changes in epigenetic regulation and metabolic transcriptomic signatureseLifeJanuary 30, 2023Nazir M Khan, Martha Elena Diaz-Hernandez, Samir Chihab, Priyanka Priyadarshani, Pallavi Bhattaram, Luke J Mortensen, Rosa M Guzzo, Hicham Drissiper the manufacturer’s instruction and fluorescence were imaged using fluorescence microscopy (BioTek Lionheart LX Automated Microscope) as described previously (Diaz-Hernandez et al., 2020). ALP staining was also performed

(4) Thermos-responsive hydrogel system encapsulated engineered exosomes attenuate inflammation and oxidative damage in acute spinal cord injuryFrontiers in bioengineering and biotechnologyJanuary 1, 2023Xiao Y, Hu X, Jiang P, Qi Z.The fluorescence of BV2 cells was observed using a Cytation 5 Cell Imaging Multimode Reader (BioTek LionheartLX) and total cellular RNA was extracted for qPCR experiments. Exos-138 uptake assay Briefly, a PKH26

(5) Structure, Biodegradation, and In Vitro Bioactivity of Zn–1%Mg Alloy Strengthened by High-Pressure TorsionMaterials (Basel)December 19, 2022Natalia Martynenko, Natalia Anisimova, Olga Rybalchenko, Mikhail Kiselevskiy, Georgy Rybalchenko, Natalia Tabachkova, Mark Zheleznyi, Diana Temralieva, Viacheslav Bazhenov, Andrey Koltygin, et al.with the plate reader. The cellular colonization was studied with an automated digital microscope (Lionheart LX, BioTek, Santa Clara, CA, USA) after staining cells with Calcein AM (Sigma-Aldrich, USA). An 18 h E. coli

(6) The Patatin–Like Phospholipase Domain Containing Protein 7 Regulates Macrophage Classical Activation through SIRT1/NF-κB and p38 MAPK PathwaysInternational Journal of Molecular SciencesNovember 29, 2022Zheng Zhao, Christoph Heier, Huimin Pang, Yu Wang, Feifei Huang, Pingan ChangAlexa Fluor® 647. Nuclei were stained with DAPI (blue). Fluorescent images were obtained with BioTek’s Lionheart LX Automated Microscope. All the presented experiments were repeated independently at least three times.

(7) Parkin regulates adiposity by coordinating mitophagy with mitochondrial biogenesis in white adipocytesNature communicationsNovember 4, 2022Moore TM, Cheng L, Wolf DM, Ngo J, Segawa M, Zhu X, Strumwasser AR, Cao Y, Clifford BL, Ma A, et al.568 overnight at 4 °C again. Images were captured under a fluorescent microscope (Zesis) and BioTek Lionheart LX automated microscope (Agilent). Quantitative RT-PCR Mouse tissue samples were homogenized using Trizol

(8) Early treatment with anti-Î±4Î²7 antibody facilitates increased gut macrophage maturity in SIV-infected rhesus macaquesFront ImmunolNovember 1, 2022Samuel D. Johnson, Lindsey A. Knight, Narendra Kumar, Omalla A. Olwenyi, Michellie Thurman, Smriti Mehra, Mahesh Mohan, Siddappa N. ByrareddyMA, USA; Product #P36935) was added, and coverslips were applied. 20X images were captured using a LIONHEART LX Automated Microscope (BioTek, Santa Clara, CA) using Gen5 3.05 software. Co-localization was performed

(9) High throughput screening of mesenchymal stem cell lines using deep learningScientific ReportsOctober 20, 2022Gyuwon Kim, Jung Ho Jeon, Keonhyeok Park, Sung Won Kim, Do Hyun Kim, Seungchul Leewith DIVA software. Imaging and preprocessing Live-cell microscopic imaging was performed using the Lionheart LX automated microscope (BioTek, Winooski, VT, USA) at 37 °C under 5% (v/v) CO2. Images were acquired using

(10) Vascular bifurcation influences the protein corona composition on nanoparticles and impacts their cellular uptakeNanoscale AdvancesJune 14, 2022Sridevi B. Conjeevaram, Ryan M. Blanchard, Amulya Kadaba, Isaac M. Adjeidye, and fixed in 4% paraformaldehyde before imaging. Cell imaging The 4T1 cells were imaged using a Lionheart LX automated microscope (Biotek, Winooski, VT) using the filter cubes corresponding to Texas red channel

(11) Construction of Enzyme-Responsive Micelles Based on Theranostic Zwitterionic Conjugated Bottlebrush Copolymers with Brush-on-Brush Architecture for Cell Imaging and Anticancer Drug DeliveryMoleculesMay 7, 2022Fangjun Liu, Dun Wang, Jiaqi Wang, Liwei Ma, Cuiyun Yu, Hua Weiby a UV−Vis spectrometer. 3.14. In Vitro Cellular Uptake Cell imaging was investigated by a BioTek LIONHEART/LX automated microscope. Bel-7402 cells were seeded with the density of 5 × 105 cells per well and incubated

(12) Safety, efficacy and delivery of multiple nucleoside analogs via drug encapsulated carbon (DECON) based sustained drug release platformEuropean Journal of Pharmaceutics and BiopharmaceuticsApril 1, 2022Tejabhiram Yadavalli, Joshua Ames, David Wu, Benjamin Ramirez, Navya Bellamkonda, Deepak Shuklacells after the virus incubation. At 24 hours post infection (hpi), cells were imaged using BioTek Lionheart LX instrument, followed by their processing for plaque assay analysis or flow cytometry analysis. 2.6

(13) Tgfbr2 in Dental Pulp Cells Guides Neurite Outgrowth in Developing TeethFrontiers in Cell and Developmental BiologyFebruary 21, 2022Monica Stanwick et al.using a standard protocol. Nuclei were stained with hematoxylin and slides were mounted for imaging. A Lionheart LX microscope (BioTek, Winooski, VT, United States) was used for imaging at ×10 magnification. IHC Images

(14) A Proximity Ligation Method to Detect Proteins Bound to Single-Stranded DNA after DNA End Resection at DNA Double-Strand BreaksMethods and ProtocolsDecember 29, 2021Faith C. Fowler, Jessica K. Tyleras reduces bias introduced when counting foci by eye. If access to a microscope such as the BioTek Lionheart LX is not feasible, images taken on any microscope can be processed and opened in ImageJ and foci can be

(15) Stringent Base Specific and Optimization-Free Multiplex Mediator Probe ddPCR for the Quantification of Point Mutations in Circulating Tumor DNACancers (Basel)November 16, 2021Franziska Schlenker et al.fluorescence color channels, for the readout of the 4-plex MP ddPCR assay, a standard fluorescence microscope (LionheartLX, Biotek, Winooski, VT, USA) was used. The workflow for droplet readout with a customized chip holder

(16) Prophylactic treatment with BX795 blocks activation of AKT and its downstream targets to protect vaginal keratinocytes and vaginal epithelium from HSV-2 infectionAntiviral researchOctober 1, 2021Madavaraju K, Yadavalli T, Singh SK, Qatanani F, Shukla D.Fluorescent Microscopy: Fluorescent images taken in this study were procured using a fluorescence microscope Lionheart LX (BioTek). This imaging system comes with an automated focusing system that can be calibrated to adjust

(17) Skeletal Deformities in Osterix-Cre;Tgfbr2f/f Mice May Cause Postnatal DeathGenes (Basel)June 25, 2021Kara Corps, Monica Stanwick, Juliann Rectenwald, Andrew Kruggel, Sarah B. Petersimaging using Lionheart a Biotek LX fluorescent microscope (n = 3 mTmG 1 atmice; 4 weeks 2 at 7and weeks n=5 Lionheart LX fluorescent microscope (n = 3mice; mTmG 1 at and 4 weeks 2 at and 7 weeks Osx-Cre;mTmG mice; 2 atmice; 4

(18) Tumor-derived IL-6 and trans-signaling among tumor, fat, and muscle mediate pancreatic cancer cachexiaThe Journal of experimental medicineJune 7, 2021Rupert JE, Narasimhan A, Jengelley DHA, Jiang Y, Liu J, Au E, Silverman LM, Sandusky G, Bonetto A, Cao S, et al.using Prolong Gold Antifade mounting media. SDH intensity was measured using color brightfield on the Lionheart LX Automated Microscope (Biotek). Visualization of C2C12 myotubes, muscle fiber dystrophin, and muscle

(19) Centrifugal Microfluidic Integration of 4-Plex ddPCR Demonstrated by the Quantification of Cancer-Associated Point MutationsProcessesJanuary 5, 2021Franziska Schlenker, Elena Kipf, Nadine Borst, Nils Paust, Roland Zengerle, Felix von Stetten, Peter Juelg, Tobias Hutzenlaub1C, 1C,the thefour-plex four-plexddPCR ddPCRworkflow workflowincluded includedthe thefluorescence fluorescence As imagingof ofeach eachddPCR ddPCRunit unitwith withaaLionheart LionheartLX LX(Biotek) (Biotek)microscope. microscope.For Forimaging, imaging,each each imaging ddPCRunit unitwas was scanned individually a

(20) hnRNPK knockdown alleviates NLRP3 inflammasome priming by repressing FLIP expression in Raw264.7 macrophagesRedox ReportDecember 3, 2020Junxia Feng, Hongyan Li, Jingchun Li, Ping Meng, Lina Wang, Chunli Liu, Shili Zhao, Wei Sun, Yunfang Zhang6-Diamidino-2-phenylindole (DAPI). The stained images were observed using an Inverted/Fluorescence Microscope (LIONHEART LX, BioTek). Cell transfection Three different small interfering RNA sequences targeting hnRNPK (sihnRNPK-1: 5

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Agilent BioTek Lionheart LX Automated Microscope from Agilent Technologies

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Agilent BioTek Lionheart LX Automated Microscope from Agilent Technologies

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