Description
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- IL-18 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- IL-18 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-18 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-18 can be calculated.
Background: Interleukin-18 (IL18), also known as interferon-gamma inducing factor, in humans is encoded by the IL18 gene, this gene contains 6 exons, and maps to 11q22.2-q22.3. It is a cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. It works by binding to the interleukin-18 receptor, and together with IL-12 it induces cell-mediated immunity following infection with microbial products like lipopolysaccharide (LPS). IL-18 has been implicated as an inflammatory mediator of Hashimoto's thyroiditis, the most common cause of autoimmune hypothyroidism. IL-18 is also able to induce severe inflammatory reactions, which suggests its role in certain inflammatory disorders