Description
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Galectin-1 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-GALECTIN-1 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Galectin-1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Galectin-1 can be calculated.
Background: Galectin-1 is a member of the galectins, which are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. In Mouses it is encoded by the LGALS1 gene, which maps to 22q12-q13. It is expressed by the endometrial stromal cells throughout the menstrual cycle, however significantly increases during implantation. The Galectin-a plays a role in the immunosuppression required for a successful pregnancy, and it may also act as an autocrine negative growth factor that regulates cell proliferation