Description
For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
INTENDED USE This PP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat PP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
PRINCIPLE OF THE ASSAY PP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PP antibody and an PP-HRP conjugate. The assay sample and buffer are incubated together with PP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color isinversely proportional to the PP concentration since PP from samples and PP-HRP conjugate compete for the anti-PP antibody binding site. Since the number of sites is limited, as more sites are occupied by PP from the sample, fewer sites are left to bind PP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PP concentration in each sample is interpolated from this standard curve