Description
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IGFBP-3 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IGFBP-3 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IGFBP-3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IGFBP-3 can be calculated.
Background: Insulin-like growth factor-binding protein 3, also known as IGFBP3, is a member of the insulin-like growth factor-binding protein (IGFBP) family. This protein has an IGFBP domain and a thyroglobulin type-I domain. It forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. It plays a role in controlling angiogenesis and cell responses in the human corpus luteum by autocrine/paracrine mechanisms. IGFBP3 protein levels decrease during the progression of prostate cancer from benign prostatic hyperplasia (BPH) to its metastatic form