Description
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-2 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-2 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-2 can be calculated.
Background: Interleukin-2 (IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression. It can act as a growth hormone for both B and T lymphocytes. IL-2 has a well documented role in induction of pruritus, furthermore, it has been found to be higher in pruritic lesions of psoriasis compared to non-pruritic ones