Description
Intended Uses: Rat uPA activity assay is intended for the quantitative determination of active urokinase plasminogen activator in rat plasma. For research use only.
Principle of the Assay: Functionally active uPA will bind to the biotinylated human PAI-1 coated on the microtiter plate. Only free active enzyme will react with the PAI-1 on the plate. Inactive or complexed enzyme will not be detected. After appropriate washing steps, anti-rat uPA primary antibody binds to the captured enzyme. Excess antibody is washed away and bound monoclonal antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of uPA