Description
The Eagle Biosciences Leptin Sensitive ELISA is a so-called Sandwich-Assay using two specific and high affinity antibodies. The Leptin in the samples binds to the first antibody coated on the microtiter plate. In the following step the second specific anti-Leptin-Antibody binds in turn to the immobilised Leptin. The second antibody is biotinylated and will be applied in a mixture with a Streptavidin-Peroxidase-Enzyme Conjugate. In the closing substrate reaction the turn of the colour will be catalysed quantitatively depending on the Leptin-level of the samples