Description
The Eagle Biosciences ELISA for IGFBP-1 is a so-called Sandwich-Assay. It utilizes two specific and high affinity antibodies for this protein. The IGFBP-1 in the sample binds to the immobilized first antibody on the microtiter plate. In the following step, the biotinylated and Streptavidin- Peroxidase conjugated second specific anti-IGFBP-1-Antibody binds in turn to the immobilised IGFBP-1. Finally, the bound peroxidase catalyses the substrate reaction resulting in a colored product. Therefore colour intensity is highly specific and quantitatively depending on the IGFBP-1-level of the samples