Flavin Adenine Dinucleotide (FAD) Assay Kit ab204710 from Abcam

Product Specs
ItemFlavin Adenine Dinucleotide (FAD) Assay Kit
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab204710
Quantity100 Test
Sample TypeSerum, Plasma, Cell Lysate, Cell culture media, Tissue Lysate
SensitivityLess than 1 nmol/well

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) NR4A3 alleviates diabetic neuropathy via GLS2-mediated mitochondrial repair and Schwann cell differentiationiScienceMay 15, 2026Bo Pang, Siyuan Chen, Yikai Bai, Yan Zhang, Zhida WangChina); ATP Assay Kit (Byotime, Cat# S0026, China); Flavin Adenine Dinucleotide Assay Kit (Abcam, Cat# ab204710, China); Coenzyme I NAD (H) Content Test Kit (Nanjing Jiancheng, Cat# A114, China); Mitochondrial DNA Isolation Kit

(2) Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosisNat Struct Mol BiolMarch 13, 2026Kirandeep K. Deol, Cynthia A. Harris, Sydney J. Tomlinson, Colin J. Delaney, Amr Al-Farhan, Alyssa J. Mathiowetz, Cody E. Doubravsky, Derek A. Pratt, James A. Olzmannbuffer. All samples were prepared for FAD fluorescence measurements using the FAD assay kit (Abcam, ab204710) according to the manufacturer’s protocol. FAD concentrations were calculated using a standard curve

(3) FAD synthase confers ferroptosis resistance and restrains CD8+ T cell recruitment in hepatocellular carcinomaNat CommunOctober 29, 2025Jiashuo Chao, Yuan Liang, Hao Wang, Ziyu Xun, Shanshan Wang, Zhengfeng Xuan, Mingming Wang, Qiyuan Huang, Rui Zhang, Mu Liu, et al.China). Measurement of flavin adenine dinucleotide (FAD) FAD levels were quantified using the FAD Assay Kit (Abcam, #ab204710) following the manufacturer’s protocol. FAD functions as a cofactor for an oxidase, generating a product

(4) Flavin affinity for the reductase HpaC differentially sensitizes Neisseria gonorrhoeae during Type IV pilus-dependent killingPLoS PathogOctober 27, 2025Linda I. Hu, Egon A. Ozer, H. S. Seifertgenerating a product that a probe detects, resulting in either fluorescence or a change in color (Abcam ab204710). We used the kit according to manufacturer provided protocol except at room temperature and with half

(5) Lysine-specific demethylase 1 (LSD1) suppresses cellular senescence by riboflavin uptake-dependent demethylation activitySci RepFebruary 23, 2025Taiichi Osumi, Taiki Nagano, Tetsushi Iwasaki, Jotaro Nakanishi, Kazuyuki Miyazawa, Shinji Kamada5′-GGCAGAGCTGGGGCTTATC-3′). FAD measurement FAD measurement was performed using the FAD Assay Kit (ab204710; Abcam, Cambridge, UK) according to the manufacturer’s instructions. Briefly, the cells were lysed and

(6) Mitochondrial-cytochrome c oxidase II promotes glutaminolysis to sustain tumor cell survival upon glucose deprivationNat CommunJanuary 2, 2025Yong Yi, Guoqiang Wang, Wenhua Zhang, Shuhan Yu, Junjie Fei, Tingting An, Jianqiao Yi, Fengtian Li, Ting Huang, Jian Yang, et al.levels The cellular FAD levels were measured using the Flavin Adenine Dinucleotide (FAD) Assay Kit (Abcam, ab204710). Cells (1 × 106) were harvested and resuspended in 400 µL ice-cold FAD Assay Buffer, then homogenized

(7) The immune response to RNA suppresses nucleic acid synthesis by limiting ribose 5-phosphateThe EMBO journalJuly 1, 2024Bhattacharjee P, Wang D, Anderson D, Buckler JN, de Geus E, Yan F, Polekhina G, Schittenhelm R, Creek DJ, Harris LD, et al.oxidase assay by the quantification of a fluorescent (OxiRed) probe using a commercial assay kit (Abcam ab204710) following the manufacturer protocol. Levels of the reduced and oxidized form of nicotinamide adenine

(8) LSD1 inhibition circumvents glucocorticoid-induced muscle wasting of male miceNature communicationsApril 26, 2024Cai Q, Sahu R, Ueberschlag-Pitiot V, Souali-Crespo S, Charvet C, Silem I, Cottard F, Ye T, Taleb F, Metzger E, et al.measurement The assessment of FAD levels in skeletal muscles was conducted utilizing the FAD Assay Kit (ab204710, Abcam) in accordance with the manufacturer’s guidelines. Briefly, skeletal muscles were lysed in ice-cold

(9) Autofluorescence is a biomarker of neural stem cell activation stateCell Stem CellApril 4, 2024Christopher S. Morrow, Kelsey Tweed, Sabina Farhadova, Alex J. Walsh, Bo P. Lear, Avtar Roopra, Ryan D. Risgaard, Payton C. Klosa, Zachary P. Arndt, Ella R. Peterson, et al.nm. FAD Assay Abcam’s FAD Quantification kit was used to measure FAD levels in qNSCs and aNSCs (Abcam ab204710) following the manufacturer’s protocols. Briefly, NSCs were pelleted and then resuspended in FAD assay

(10) Chronic stress alters hepatic metabolism and thermodynamic respiratory efficiency affecting epigenetics in C57BL/6 miceiScienceMarch 15, 2024Aleksandra Nikolic, Pia Fahlbusch, Nele-Kathrien Riffelmann, Natalie Wahlers, Sylvia Jacob, Sonja Hartwig, Ulrike Kettel, Martina Schiller, Matthias Dille, Hadi Al-Hasani, et al.Assay,",Promega,Cat#G9071 "MTase-Glo Methyltransferase assay,",Promega,Cat#V7601 FAD+ (FAD Assay Kit),Abcam,Cat#Ab204710 "SIRT-Glo Assay System, Promega, Germany",Promega,Cat#G4650 Mouse Gene (MTA) arrays,Thermo Fisher

(11) A familial natural short sleep mutation promotes healthy aging and extends lifespan in DrosophilaResearch SquareJune 30, 2023Pritika Pandey, P. Kerr Wall, Stephen R. Lopez, Olga S. Dubuisson, Elizabeth R.M. Zunica, Wagner S. Dantas, John P. Kirwan, Christopher L. Axelrod, Alyssa E. JohnsonActivity FAD activity was determined colorimetrically by commercially available enzymatic assay (Abcam, ab204710). Isolated thorax muscles (~5/biological replicate) were deproteinated (Abcam, ab204708), homogenized

(12) Broad responses to chemical adducts shape the natural antibody repertoire in early infancyScience advancesMay 12, 2023Mashiko S, Shihab RR, See SB, Schahadat LGZ, Aguiar TFM, Roy P, Porcheray F, Zorn E.quantification of FAD, NAD, and MDA, the following kits were used: Flavin Adenine Dinucleotide Assay Kit (ab204710) and NAD/NADH Assay Kit (ab65348) (Abcam, Cambridge, MA, USA), as well as OxiSelect MDA Adduct Competitive

(13) Riboflavin transporter SLC52A1, a target of p53, suppresses cellular senescence by activating mitochondrial complex IIMolecular biology of the cellNovember 1, 2021Nagano T, Awai Y, Kuwaba S, Osumi T, Mio K, Iwasaki T, Kamada S.sample – ΔCt calibrator). FAD measurement FAD measurement was performed using the FAD Assay Kit (ab204710; Abcam, Cambridge, UK) according to the manufacturer’s instructions. Briefly, the cells were lysed and

(14) LSD1-mediated enhancer silencing attenuates retinoic acid signalling during pancreatic endocrine cell developmentNature communicationsApril 29, 2020Vinckier NK, Patel NA, Geusz RJ, Wang A, Wang J, Matta I, Harrington AR, Wortham M, Wetton N, Wang J, et al.measurements Flavin adenine dinucleotide (FAD) on hESC-derived cells was isolated with the FAD Assay Kit (Abcam, ab204710). Cell lysates were deproteinated using perchloric acid and FAD was quantified using the colorimetric

(15) Autofluorescence spectroscopy in redox monitoring across cell confluenciesPLOS ONEDecember 18, 2019Derrick Yong, Ahmad Amirul Abdul Rahim, Chaw Su Thwin, Sixun Chen, Weichao Zhai, May Win NaingNADH and FAD using the NAD/NADH-Glo™Assay Kit (G9071, Promega Corporation, USA) and FAD Assay Kit (ab204710, Abcam, United Kingdom), correspondingly. For both assays, cells were seeded at concentrations of 1.5,

(16) d-amino acid oxidase promotes cellular senescence via the production of reactive oxygen speciesLife science allianceFebruary 1, 2019Nagano T, Yamao S, Terachi A, Yarimizu H, Itoh H, Katasho R, Kawai K, Nakashima A, Iwasaki T, Kikkawa U, et al.NBD-F, the samples were injected into the HPLC. FAD assay FAD assay was performed using FAD Assay Kit (ab204710; Abcam) according to the manufacturer's instructions. Briefly, the cells were lysed and deproteinized

(17) The luminance ratio of autofluorescence in a xenograft mouse model is stable through tumor growth stagesClinical and Experimental Dental ResearchOctober 1, 2018Shigeki Sumi, Naoki Umemura, Makoto Adachi, Takahisa Ohta, Kosuke Naganawa, Harumi Kawaki, Eiji Takayama, Nobuo Kondoh, Shinichiro Sumitomomass. The tissues were floated in 400 μl of FAD buffer attached to a FAD assay kit (Abcam Technology: ab204710, Cambridge, UK) and mashed on ice using a BioMasher II 1.5‐ml tube‐size disposable homogenizer (Nippi

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Flavin Adenine Dinucleotide (FAD) Assay Kit from Abcam

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Flavin Adenine Dinucleotide (FAD) Assay Kit from Abcam

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