MojoSort™ Mouse CD4 Naïve T Cell Isolation Kit 480039 from BioLegend

Product Specs
ItemMojoSort™ Mouse CD4 Naïve T Cell Isolation Kit
CompanyBioLegend
Price $232.00Supplier Page View Company Product Page
Catalog Number480039
Quantity20 tests
ApplicationsCell Separation (MojoSort™, ) - Quality tested
FormatCocktail: Phosphate buffer solution containing 0.09% sodium azide, pH 7.2.
Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide
ReactivityMouse
Detection TargetCD4
Cell Type (standardized)CD4+ T Cell

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Extracellular ATP Functions as a Metabolic Lineage Selection Signal That Stabilizes Tc9 Cells During Adoptive T Cell TherapyInternational Journal of Molecular SciencesMarch 31, 2026Jie Ren, Zhengrong Gong, Yutong Zhong, Ruipei Xiao, Khadija Urooj, Yuan Gao, Enguang Bi, Handuo Wangsorting were performed using MojoSort™ Naïve T Cell Isolation Kits (BioLegend, San Diego, CA, USA, Cat# 480040) according to the manufacturer’s instructions. Briefly, for every 1 × 107 total cells, 10 μL of antibody

(2) The MEK1/2-IRF4 axis fosters T follicular helper cell differentiationand antitumor humoral immune responseJournal of Translational MedicineMarch 16, 2026Shuan Ran, Song Wang, Ran Li, Longyong Lai, Jizhang Yu, Xi Zhang, Yuan Li, Weicong Ye, Junjie Zong, Xiaohan Li, et al.single-cell suspensions via positive selection using the MojoSort™ Mouse CD4 Naïve T Cell Isolation Kit (#480039, Biolegend). The cell suspensions were washed twice with cold PBS supplemented with 2% fetal bovine

(3) Proinflammatory keratinocytes drive a novel mouse model of autoimmunity with systemic and cutaneous lupus erythematosusImmun InflammFebruary 3, 2026Jingru Tian, Liqing Shi, Dingyao Zhang, Xu Yao, Jun Lu, Ming Zhao, Qianjin Luco-culture CD4+ naive T cells were isolated from mouse spleens using a MACS cell separation system (BioLegend #480040). For dendritic cells, we used flow cytometry to exclude potential contaminating cells, followed by

(4) A fungal-derived cyclic peptide enhances Th9-mediated antitumor immunity by targeting ZAP70 and SREBP1Journal of Clinical InvestigationFebruary 2, 2026Wenli Zhao, Yang Zhou, Yuyang Chen, Yicheng Sun, Jiaxin Tang, Yihan Zhu, Jie Ren, Tianxu Du, Handuo Wang, Yuan Gao, et al.C57BL/6J or CD45.1+ OT-II mice using the MojoSort Mouse CD4 Naive T Cell Isolation Kit (BioLegend, catalog 480040), following the manufacturer’s protocol. Purified cells were cultured in RPMI-1640 medium supplemented

(5) Tumor-initiating stem cells fine-tune the plasticity of neutrophils to sculpt a protective nicheCancer CellJanuary 12, 2026Weijie Guo, Jingyun Luan, Xuejie Huang, Daniel Leon, Sophie Gang, Benjamin Nicholson, Breanna Bertacchi, Diana Bolotin, Mark W. Lingen, Alexander T. Pearson, et al.MojoSort Mouse Ly-6G Selection Kit,Biolegend,Cat# 480124 MojoSort Mouse CD4 NaiveT Cell Isolation Kit,Biolegend,Cat# 480040 RNAscope Multiplex Fluorescent Reagent Kit v2,Advanced Cell Diagnostics,Cat# 323100 BD Cytofix/Cytoperm™

(6) IL-2/IL-15 signaling induces NK cell production of FLT3LG augmenting anti-PD-1 immunotherapyCancer Immunology ResearchJanuary 8, 2026Shayan C. Avanessian, Renske J.E. van den Bijgaart, Nayvin W Chew, Valentina M. Supper, Thao T. Tang, Yuzheng Zhang, Ying-Qi Zhao, Kokoro Abe, Jordan Gauthier, Kevin C. Barrymice and processed as above using MojoSort™ Mouse CD4 Naïve T Cell Isolation Kit (Biolegend, Cat. No. 480040) and MojoSort™ Mouse CD8 T Cell Isolation Kit (Biolegend, Cat. No. 480044). Baseline media was made

(7) Suxiao Jiuxin Pill Promotes Post-Myocardial Infarction Cardiac Repair by Enhancing Regulatory T Cell Function via the ST2L Signaling PathwayJournal of Inflammation ResearchJanuary 6, 2026Lusha Zhang, Ying Guo, Yuming Fan, Ting Liu, Tianyu Wang, Mengyao Li, Shaoxia Wang, Qi Yu, Hong Wangstrainer. Spleen Naïve CD4+ T cell was sorted with a MojoSort™ Mouse CD4 Naïve T Cell Isolation Kit (480039, BioLegend, USA) according to the kit’s instructions. Purity of isolated cells was then measured by

(8) Antibody-induced internalization and degradation of PLA2R amplifies CD4+ T cell activationTheranosticsJanuary 1, 2026Yuning Liu, Yinyin Li, Hui Zhou, Jieli Yu, Pei Deng, Weiwei Xu, Bingqian Pan, Lei Zhang, Hong Zhou, Jing Zhang, et al.T cell isolation. Naive CD4+ T cells were isolated using MojoSort Mouse CD4 Naïve T Cell Isolation Kit (480040, Biolegend) according to the manufacturer instruction. 1 x 105 CD4+ T cells labeled with 5 μM CFSE (Thermo Fisher Scientific

(9) Single-cell multiomics reveals macrophage-derived IL-23 and CXCL9/10 drive pathogenic IFNG + IL17 + T cells in immunotherapy-related colitisJournal for ImmunoTherapy of CancerNovember 29, 2025Zhongshun Tang, Jiaolong Shi, Shuai Xu, Sheng Liu, Wenqian Yin, Yuyang Xiang, Junlin Li, Zhanke He, Xingxing Yao, Zhou Li, et al.splenocytes of C57BL/6J mouse with the MojoSort Mouse CD4 Naïve T Cell Isolation Kit (Biolegend, Cat# 480040). For Th17 polarization, isolated cells were cultured under activating conditions with plate-immobilized

(10) The deubiquitinase USP17LA negatively regulates T-cell activation and attenuates anti-tumor immunityEMBO ReportsSeptember 29, 2025Huiling Zhang, Zhihan Guo, Gaigai Wei, Jingjing Yi, Zixi Wang, Yuqi Zhang, Haiping Zhao, Tingrong Ren, Yihan Wang, Jiating Kuang, et al.MojoSort™ Mouse CD3 T Cell Isolation Kit,BioLegend,480031 MojoSort™ Mouse CD4 Naive T Cell Isolation Kit,BioLegend,480040 MojoSort™ Mouse CD8 Naive T Cell Isolation Kit,BioLegend,480044 DMEM,Sangon Biotech,E600003-0500 RPMI

(11) Phosphoglycerate mutase regulates Treg differentiation through control of serine synthesis and one-carbon metabolismeLifeJuly 28, 2025Godfrey WH, Lee JJ, Shanmukha S, Cho K, Deng X, Ambati CSR, Putluri V, Mostafa Kamal AH, Kim PM, Putluri N, et al.isolated by negative selection using the MojoSort CD4 + naïve T-cell isolation kit from Biolegend (Catalog 480040) according to the manufacturer’s instructions. Plates were coated overnight at 4 °C with plate-bound

(12) The OGT–TFF2 axis mediates intrahepatic crosstalk and MASH pathogenesisHepatologyJune 30, 2025Lei Zhang, Cuijuan Han, Man Mohan Shrestha, Jiamei Le, Wilhelm K. Berger, Yiheng Huang, Reina Desrouleaux, Eric Wang, Laszlo Nagy, Xiaoyong YangMojoSort™ Mouse CD8 naïve T Cell Isolation Kit,Biolegend,480043 MojoSort™ Mouse CD4 Naïve T Cell Isolation Kit,Biolegend,480039 Dual-Glo® Luciferase Assay System,Promega Corporation,E2920 LEGEND MAX™ Mouse IL-17A ELISA Kit Pre-coated

(13) Protocol for CRISPR-mediated deletion of cis-regulatory element in murine Th17 cells for in vivo assessment of effector functionSTAR ProtocolsJune 20, 2025Xiancai Zhong, Hongmin Wu, Guanpeng Wang, Zuoming Sunassays,Critical commercial assays ,, MojoSort Mouse CD4 Naïve T Cell Isolation Kit,BioLegend,Cat# 480040 MOG35-55/CFA Emulsion PTX,Hooke Laboratories,Cat# EK-2110 BD Pharmingen Transcription-Factor Buffer Set

(14) Pre-existing epigenetic state and differential NF-κB activation shape type 2 immune cell responsesImmunityJune 10, 2025Vincent Guichard, Felipe Batista Leão, Jingyao Zhao, Yingyu Zhang, Takamasa Ito, Simon Shirley, Thomas S. Postler, Ruxiao Tian, Yuefeng Huang, Sankar GhoshPowerTrack™ SYBR Green Master Mix for qPCR,Thermo Scientific,Cat# A46113 MojoSort™ Mouse CD4 Naïve T Cell Isolation Kit,Biolegend,Cat# 480040 RNeasy Mini Kit,QIAGEN,Cat# 74104 RNeasy Micro Kit,QIAGEN,Cat# 74004 MinElute PCR Purification Kit,QIAGEN,Cat#

(15) CXXC-finger protein 1 associates with FOXP3 to stabilize homeostasis and suppressive functions of regulatory T cellsElifeApril 4, 2025Xiaoyu Meng, Yezhang Zhu, Kuai Liu, Yuxi Wang, Xiaoqian Liu, Chenxin Liu, Yan Zeng, Shuai Wang, Xianzhi Gao, Xin Shen, et al.(MOG35-55-specific TCR transgenic) mice were isolated using the Mouse CD4 Naive T cell Isolation Kit480039 (480039; BioLegend). As previously described (Chou et al., 2021), 2D2 naive CD4+ T cells alone (5 × 105 per

(16) KLHL25-ACLY module functions as a switch in the fate determination of the differentiation of iTreg/Th17Commun BiolMarch 21, 2025Miaomiao Tian, Fengqi Hao, Xin Jin, Xinyu Wang, Tianyi Chang, Shuang He, Huiyue Wang, Ying Jiang, Yang Wang, Jia Liu, et al.from the spleens of 6- to 8-week-old mice using the MojoSort™ Mouse CD4+ Naïve T Cell Isolation Kit (480040, Biolegend) according to the manufacture’s instruction. For Th0 induction, 2–3 × 105 naïve CD4+ T cells

(17) TMEM41B is an endoplasmic reticulum Ca2+ release channel maintaining naive T cell quiescence and responsivenessCell DiscovMarch 4, 2025Yuying Ma, Yi Wang, Xiaocui Zhao, Gang Jin, Jing Xu, Zhuoyang Li, Na Yin, Zhaobing Gao, Bingqing Xia, Min PengNaive CD4 T cells were purified with MojoSort™ Mouse CD4 Naive T Cell Isolation Kit (BioLegend, Cat# 480040) according to the manufacturer’s instructions. Freshly isolated naive T cells were seeded on XF96 microplates

(18) CD4+ T cell–innate immune crosstalk is critical during Staphylococcus aureus craniotomy infectionJCI InsightFebruary 24, 2025Gunjan Kak, Zachary Van Roy, Rachel W. Fallet, Lee E. Korshoj, Tammy Kielianisolated from the spleens of 6- to 8-week-old mice using a MojoSort mouse naive T cell isolation kit (480040, BioLegend). T cells were cultured in RPMI supplemented with 10% FBS (HyClone-Cytiva), penicillin/streptomycin/fungizone

(19) Interferon-gamma receptor signaling regulates innate immunity during Staphylococcus aureus craniotomy infectionJ NeuroinflammationFebruary 22, 2025Zachary Van Roy, Gunjan Kak, Rachel W. Fallet, Tammy Kielianthrough 70 μm nylon mesh before isolation using a MojoSort mouse naïve T cell isolation kit (Cat. #480040, BioLegend). T cells were cultured in RPMI supplemented with 10% FBS, penicillin/streptomycin/fungizone,

(20) Fine-tuning levels of filamins a and b as a specific mechanism sustaining Th2 lymphocyte functionsNature CommunicationsDecember 5, 2024Kilian Maire, Léa Chamy, Samira Ghazali, Manon Carratala-Lasserre, Margot Zahm, Clément Bouisset, Arnaud Métais, Lucie Combes-Soia, Lidia de la Fuente-Vizuete, Hussein Trad, et al.naive CD4+ cells were isolated from spleen immunocytes of mice using a CD4 naive T-cell Isolation Kit (#480040; BioLegend) and stimulated for 3 days with plate-bound anti-CD3ε (5 µg/ml) and were differentiated in

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MojoSort™ Mouse CD4 Naïve T Cell Isolation Kit from BioLegend

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MojoSort™ Mouse CD4 Naïve T Cell Isolation Kit from BioLegend

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