Sample Activation Kit 1 DY010 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemSample Activation Kit 1
CompanyR&D Systems, a Bio-Techne Brand
Price $130.00Supplier Page View Company Product Page
Catalog NumberDY010
Quantity1 Kit
ApplicationsELISA
ReactivityMulti-Species
StorageStore the unopened product at room temperature. Do not use past expiration date

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Serglycin modulates inflammation and metabolism in macrophagesiScienceApril 17, 2026Shirin Porteymour, Saikat Das Sajib, Susannah von Hofsten, Atanaska I. Doncheva, Christina D. Bjørnvall, Beate Hegge, Mélina Gautier, Mirjana Grujic, Pratibha Kolan, Karthickeyan Chella Krishnan, et al.(Cat. No. DY278), IL6 (Cat. No. DY206), IL-1β (Cat. No. DY201), TGF-β (Cat. No. DY240; activation kit DY010), and TNF-α (Cat. No. DY210), following the manufacturer’s instructions. Absorbance was recorded using

(2) The Function of Transforming Growth Factor 2 in Facilitating Inflammasome Activation to Enhance the Development of Myopia via Complement SystemCellsAugust 20, 2025Sheng-Chun Lin, Yu-An Hsu, Chi-Fong Lin, Chih-Sheng Chen, Peng-Tai Tien, Yao-Chien Wang, Ching-Yao Chang, En-Shyh Lin, Jamie Jiin-Yi Chen, Ming-Yen Wu, et al.tissues were homogenized; 50 μg total protein was subjected to treatment with Sample Activation 1 (DY010; R&D systems, Minneapolis, MN, USA). The TGF-β2 concentration in the tissue lysate was determined by

(3) Creatine/creatinine ratio and myostatin as biomarkers to monitor muscle function in Duchenne Muscular Dystrophy patientsmedRxivAugust 14, 2025Chiara Degan, Roula Tsonaka, Sharon I. de Vries, Nadine Ikelaar, Menno van der Holst, Hermien E. Kan, Erik H. Niks, Pietro SpitaliQuantikine ELISA kit (Bio-Techne #DGDF-80) in combination with a sample activation kit (BioTechne #DY010) and a quality control set 923 (BioTechne #QC98). In brief, 30 μl of serum was activated and subsequently

(4) Blood-based biomarkers for early frailty are sex-specific: validation of a combined in silico prediction and data-driven approachGeroScienceJune 1, 2025de Jong JCBC, Caspers MPM, Dulos R, Snabel J, van der Hoek MD, van der Leij FR, Kleemann R, Keijer J, Nieuwenhuizen AG, van den Hoek AM, et al.activated to measure the concentration of total myostatin using a sample activation kit (R&D systems, DY010). Statistical analysis For the statistical analysis of biomarker serum concentration data, participants

(5) NF-κB/RelA signaling in secretoglobin progenitors mediates plasticity and MMP-induced barrier disruption in house dust mite-induced allergic asthmaAmerican Journal of Physiology-Lung Cellular and Molecular PhysiologyJuly 1, 2024Melissa E. Skibba, Allan R. BrasierDY406), and Mouse TGF-β1 DuoSet ELISA Kit (R&D Systems; DY1679) along with the Sample Activation Kit 1 (DY010) to detect active TGF-β1. These assays were performed according to the manufacturer’s instructions.

(6) Inhibition of TGFβ1 activation prevents radiation‐induced lung fibrosisClinical and Translational MedicineJanuary 18, 2024Minxiao Yi, Ye Yuan, Li Ma, Long Li, Wan Qin, Bili Wu, Bolong Zheng, Xin Liao, Guangyuan Hu, Bo Liuwere measured using a TGFβ1 Quantikine ELISA Kit (MB100B, R&D Systems) and a sample activation kit (DY010, R&D Systems) according to the manufacturer's instructions. 2.13 Statistical analysis For comparisons

(7) TGF-β broadly modifies rather than specifically suppresses reactivated memory CD8 T cells in a dose-dependent mannerProceedings of the National Academy of SciencesNovember 28, 2023Alexis Taber, Andrew Konecny, Shannon K. Oda, James Scott-Browne, Martin Prlicdetermined by acid activation of the latent TGF-β1 in the sample using the sample activation kit 1 (DY010) (R&D Systems). RNA-sequencing. Bulk RNA-seq was performed on 500 sort-purified OT-I T cells derived

(8) Efficacy of combined tumor irradiation and K(Ca)3.1-targeting with TRAM-34 in a syngeneic glioma mouse modelScientific reportsNovember 23, 2023Stransky N, Ganser K, Quintanilla-Martinez L, Gonzalez-Menendez I, Naumann U, Eckert F, Koch P, Huber SM, Ruth P.DuoSet ELISA Ancillary Reagent Kit 1 and Sample Activation Kit 1 (all R&D Systems, DY1679-05, DY007B, DY010) according to the manufacturer’s instruction. Samples were prepared by plating 187.500 cells/1 mL medium

(9) Analysis of proteins released from osteoarthritic cartilage by compressive loadingScientific reportsOctober 25, 2023Tsuno H, Tanaka N, Naito M, Ohashi S, Iwasawa M, Kadoguchi T, Mitomi H, Matsui T, Furukawa H, Fukui N.

(10) Long-Term Effects of Pulmonary Endarterectomy on Right Ventricular Stiffness and Fibrosis in Chronic Thromboembolic Pulmonary HypertensionCirculation: Heart FailureOctober 1, 2023Natalia J. Braams, Azar Kianzad, Jessie van Wezenbeek, Jeroen N. Wessels, Samara M.A. Jansen, Stine Andersen, Anco Boonstra, Esther J. Nossent, J. Tim Marcus, Ahmed A. Bayoumy, et al.protein concentrations using the human TGF- β Duo set ELISA kit (DY240) and Sample activation Kit 1 (DY010; R & D Systems Inc., Minneapolis, MN) per manufacturer’s protocol. Reference values were derived from

(11) Long-Term Effects of Pulmonary Endarterectomy on Right Ventricular Stiffness and Fibrosis in Chronic Thromboembolic Pulmonary HypertensionCirc Heart FailSeptember 7, 2023Natalia J. Braams, Azar Kianzad, Jessie van Wezenbeek, Jeroen N. Wessels, Samara M.A. Jansen, Stine Andersen, Anco Boonstra, Esther J. Nossent, J. Tim Marcus, Ahmed A. Bayoumy, et al.protein concentrations using the human TGF- β Duo set ELISA kit (DY240) and Sample activation Kit 1 (DY010; R & D Systems Inc., Minneapolis, MN) per manufacturer’s protocol. Reference values were derived from

(12) TGF-β broadly modifies rather than specifically suppresses reactivated memory CD8 T cells in a dose-dependent mannerbioRxiv : the preprint server for biologyJuly 29, 2023Taber A, Konecny A, Scott-Browne J, Prlic M.determined by acid activation of the latent TGF-β1 in the sample using the sample activation kit 1 (DY010) (R&D Systems). Bulk RNA Sequencing Bulk RNA-seq was performed on 500 sort-purified OT-I T cells derived

(13) Anti-Fibrotic and Anti-Inflammatory Role of NO-Sensitive Guanylyl Cyclase in Murine LungInternational journal of molecular sciencesJuly 19, 2023Englert N, Burkard P, Aue A, Rosenwald A, Nieswandt B, Friebe A.the manufacturer’s instructions. The undiluted samples were activated using a sample activation kit (DY010; R&D Systems, Minneapolis, MN, USA). Duplicates of standards and samples were transferred to a 96-well

(14) Longitudinal Assessment of Creatine Kinase, Creatine/Creatinineratio, and Myostatin as Monitoring Biomarkers in Becker Muscular DystrophyNeurologyFebruary 28, 2023Nienke M. van de Velde, Zaïda Koeks, Mirko Signorelli, Nisha Verwey, Maurice Overzier, Jaap A. Bakker, Gautam Sajeev, James Signorovitch, Valeria Ricotti, Jan Verschuuren, et al.myostatin (pg/mL) was performed by ELISA using a kit (cat. n. DGDF80) including the activation kit (cat. n. DY010) and quality control set 794 (cat. n. QC98). All products were obtained from R&D Systems, Bio-Techne,

(15) Longitudinal Assessment of Creatine Kinase, Creatine/Creatinineratio, and Myostatin as Monitoring Biomarkers in Becker Muscular DystrophyNeurologyFebruary 28, 2023Nienke M. van de Velde, Zaïda Koeks, Mirko Signorelli, Nisha Verwey, Maurice Overzier, Jaap A. Bakker, Gautam Sajeev, James Signorovitch, Valeria Ricotti, Jan Verschuuren, et al.myostatin (pg/mL) was performed by ELISA using a kit (cat. n. DGDF80) including the activation kit (cat. n. DY010) and quality control set 794 (cat. n. QC98). All products were obtained from R&D Systems, Bio-Techne,

(16) A TGFβR inhibitor represses keratin-7 expression in 3D cultures of human salivary gland progenitor cellsScientific reportsSeptember 2, 2022Fowler EW, van Venrooy EJ, Witt RL, Jia X.R&D Systems). For the activation of latent TGF-β1, the medium was treated with Sample Activation Kit (DY010; R&D Systems). TGF-β1 and GDF-15 ELISA were performed using Substrate Reagent Pack (DY999; R&D Systems),

(17) Exposure modality influences viral kinetics but not respiratory outcome of COVID-19 in multiple nonhuman primate speciesPLoS pathogensJuly 1, 2022Fears AC, Beddingfield BJ, Chirichella NR, Slisarenko N, Killeen SZ, Redmann RK, Goff K, Spencer S, Picou B, Golden N, et al.#BD100B) according to manufacturer’s instructions and utilizing a Sample Activation Kit 1 (R&D Systems #DY010) and Human TGF-β1 Controls (R&D Systems #QC01-1). Each animal’s necropsy BAL supernatant was compared

(18) Peripheral blood CD4(pos)CD25(pos)FoxP3(pos) cells and inflammatory cytokines as biomarkers of response in rheumatoid arthritis patients treated with CTLA4-IgArthritis research & therapyJune 15, 2022Gremese E, Tolusso B, Petricca L, Di Mario C, Gigante MR, Ferraccioli G, Alivernini S.

(19) Targeting Cell Death Mechanism Specifically in Triple Negative Breast Cancer Cell LinesInternational journal of molecular sciencesApril 26, 2022Pruteanu LL, Braicu C, Módos D, Jurj MA, Raduly LZ, Zănoagă O, Magdo L, Cojocneanu R, Paşca S, Moldovan C, et al.R&D Systems, cat no. DY008, Minneapolis, MN, USA) and Sample Activation Kit 1 (R&D Systems, cat no. DY010, Minneapolis, MN, USA). For IL6 quantification from cell culture, ELISA was performed using the IL6

(20) Fatty acid nitroalkene reversal of established lung fibrosisRedox biologyApril 1, 2022Koudelka A, Cechova V, Rojas M, Mitash N, Bondonese A, St Croix C, Ross MA, Freeman BA.then neutralized by 20 μl 1.2 M NaOH/0.5 M HEPES using Sample Activation Kit 1 reagents (R&D Systems: DY010). All media samples were diluted so that absorbance results were in the range of the standard curve.

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Sample Activation Kit 1 from R&D Systems, a Bio-Techne Brand

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R&D Systems, a Bio-Techne Brand

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Sample Activation Kit 1 from R&D Systems, a Bio-Techne Brand

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