Nano-Glo Live Cell Assay System N2012 from Promega

Product Specs
ItemNano-Glo Live Cell Assay System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberN2012
Quantity1,000 assays

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Preventing trogocytosis by cathepsin B inhibition augments CAR T-cell functionSignal Transduct Target TherApril 22, 2026Kenneth A. Dietze, Kiet Nguyen, Aashli Pathni, Frank Fazekas, Wenxiang Sun, Ethan Rosati, Jillian M. Baker, Maday Galeana Figueroa, Etse Gebru, Daniel Yamoah, et al.tumor cells were resuspended in Opti-MEM reduced serum media. Live Cell Substrate (Promega, catalog no. N2011) was prepared according to the manufacturer’s instructions. K562 tumor cells and prepared live cell

(2) Mitochondrial presequences harbor variable strengths to maintain organellar functionJournal of Cell BiologyApril 6, 2026Youmian Yan, Baigalmaa Erdenepurev, Thiago N. Menezes, Ian Collinson, Natalie M. Niemiwere purchased from MilliporeSigma. Nano-Glo Live Cell Reagent was purchased from Promega (catalog #N2012). Glutathione resin (catalog #NC1057345) and PreScission Protease (catalog #NC1539078) were purchased

(3) Nitrogen cavitation enables rapid and high-yield preparation of functional cell-membrane-derived vesiclesNanoscale AdvancesMarch 17, 2026Changting Li, Dehua Luo, Can Peng, Qun Luo, Wenjing Xu, Jincheng Zhou, Wen Su, Wei Wu, Yi-Feng Wangcell. Enzymatic activity assay of purified CMDVsHEK 293_NLuc According to the Nano Glo® Live Cell Assay kit (N2011, Promega), the substrate and buffer were mixed in a ratio of 1 : 19, then the mixture was mixed with

(4) A dual topology of STIM1 at the plasma membrane regulates calcium constitutive entryCell Mol Life SciMarch 6, 2026Paul Buscaglia, Nelig Le Goux, Patrice Hemon, Anthony Mainguy, Maela Hus, Mathieu Gimaray, Paul Roger Claude Imbert, Alix A. J. Rouault, Julien A. Sebag, Olivier Mignenand replaced by 100 μL of NanoBiT complementation reagent (Promega, Nano-Glo live cell assay system, N2012) prepared following commercial recommendations. Luminescence was measured using the FlexStation 3™ (Molecular

(5) Dual agonism and selective T-cell depletion activity of a PD-1-directed antibody for treating autoimmune diseasesMAbsFebruary 5, 2026Wenbo Jiang, Lingyun Li, Weili Xue, Xuzhi He, Xuebin Chu, Lei Song, Xue Li, Ranran Zhao, Xinghang Yuan, Xiaoliang Jin, et al.Luciferase activity in the reporter T cells was determined by a Nano-Glo live cell assay (Cat. No. N2012, Promega) following the manufacturer’s instruction, with RLU measured on a SpectraMax iD5 microplate

(6) NS2B is indispensable for NS3/NS5 interaction of Tembusu virus in living cellsPoultry ScienceFebruary 1, 2026Yu He, Yanfan Shi, Zhen Wu, Tao Wang, Mingshu Wang, Renyong Jia, Dekang Zhu, Mafeng Liu, Xinxin Zhao, Qiao Yang, et al.100 µl of fresh serum-free DMEM. Subsequently, 25 µl of Nano-Glo Live Cell Reagent was added (Cat. #N2012, Promega, Beijing, China). The plate was gently mixed, and luminescence was measured immediately usingthe

(7) Effector–host interactome map links type III secretion systems in healthy gut microbiomes to immune modulationNature MicrobiologyJanuary 26, 2026Veronika Young, Bushra Dohai, Hridi Halder, Jaime Fernandez-Macgregor, Niels S. van Heusden, Thomas C. A. Hitch, Benjamin Weller, Patrick Hyden, Deeya Saha, Daan K. J. Pieren, et al.CS3002A02) for 50 min. Luminescence was measured after addition of 25 μl fresh Nano-Glo reagent (Promega, N2012) using a SpectraMax ID3 microplate reader (1,000 ms). Each strain was tested with four technical replicates

(8) An engineered nanobody inhibitor for molecular-to-circuit control of opioid receptor functionbioRxivJanuary 18, 2026Zoé Valbret, Paul J. Lamothe-Molina, Sofia Papadogkonaki, Antoine Koehl, Jan Vincent V. Arafiles, Ziva Shapiro Shapiro Tuchman, Nicole M. Fisher, Arthur Radoux-Mergault, Annika Canziani, Gaoyang Huang, et al.plates (20,000 cells/well) in FluoroBrite DMEM (Gibco) and the NanoLuc substrate Nano-Glo (Promega, N2012), and incubated for 45 min at 37°C. The luminescence signal was recorded using the FDSS/μCELL kinetic

(9) On the same side: VISTA and its ligands interact in cis on the cell surfaceProtein SciDecember 22, 2025Karina Smorodinsky-Atias, Gil Wiseglass, Alina Artyukhova, Mariana Salem, Maya Kashani, Nadir Boni, Rachel Levy, Rotem Rubinsteintransfection, the media was replaced with 0.1 mL Opti‐MEM™ and a fresh Nano‐Glo® Live cell Reagent (Promega #N2011) was prepared in a 1:20 dilution. 20 μL of reagent was added to each well. Luminescence was measured

(10) Auranofin attenuates TOPBP1-mediated ATR replication stress response and improves chemotherapeutic response in breast tumor modelsJ Clin InvestDecember 15, 2025Shuai Ma, Yingying Han, Rui Gu, Qi Chen, Qiushi Guo, Yuan Yue, Cheng Cao, Ling Liu, Zhenzhen Yang, Yan Qin, et al.measured according to the manufacturer’s instructions using Nano-Glo Live Cell Assay System (Promega, N2012) and Synergy plate reader (BioTek Instruments). BLI. The BLI assays were performed using Octet RED96

(11) Targeted NanoBiT Screening Identifies a Novel Interaction Between SNAPIN and Influenza A Virus M1 ProteinBiology (Basel)December 11, 2025Xiaoxuan Zhang, Huanhuan Wang, Conghui Zhao, Wenjun Shi, Faxin Wen, Haoxi Qiang, Sha Liu, Peilin Li, Xinhui Chen, Chunping Zhang, et al.per well. Prior to measurement, 25 µL of diluted substrate from the Nano-Glo Live Cell Assay System (N2011, Promega, Madison, WI, USA) was added to each well, and luminescence was measured using a GloMax 96 microplate luminometer

(12) Human and viral chemokines differentially modulate G protein signaling, β-arrestin recruitment and chemotaxis mediated by the viral G protein-coupled receptor ORF74Cell Communication and SignalingNovember 22, 2025Qianqian Kong, Brent Van Bosstraeten, Maarten Jacquemyn, Dirk Daelemans, Steven De Jonghe, Dominique Schols, Tom Van Loywith assay buffer, after which 80 µL/well of a 1:200 diluted Nano-Glo® Live Cell Substrate (Promega, #N2012) working solution was added and plates were transferred into the FLIPR Penta. Plates were left to incubate

(13) Assembly and dynamic regulation of the tip filament of the Bordetella type III secretion system injectisomemBioNovember 12, 2025Ivana Malcova, Martin Zmuda, Jan Valecka, Jana Kamanovacentrifugation (5 minutes; 8,000 g) and added to cells at MOI 5:1 along with Nano-Glo Live Cell Reagent (Cat.No. N2011, Promega), containing cell-permeable luciferase substrate and Nano-Glo buffer. After centrifugation

(14) Inhibition of Histone Deacetylases Induces Cancer Cell Apoptosis Through the PERK Pathway of ER Stress ResponseJ Cell Mol MedOctober 29, 2025Xuan Wang, Caiyun Yang, Yili Yang, Feng Xu, Donglan Yuan, Yuexi Guconcentrations for 12 h. The luciferase activity was measured by using Nano‐Glo Live Cell Reagent (Promega, #N2012) according to the manufacturer's instructions. 2.4 Western Blotting Cells were lysed using RIPA buffer

(15) Quantitative cellular characterization of extracellular mitochondria uptake and deliveryNat CommunOctober 10, 2025Zahra Al Amir Dache, Maud Chevé, Julia Dancourt, Grégory Lavieudiluted in 200 μL PBS, and 20 μL of Nano-Glo™ Live Cell assay reagent (20 μL buffer + 0.5 μL substrate) (N2012, Promega, Wisconsin, USA) was added to immediately measure the remaining NLuc activity in each well

(16) Discovery of a Potent and Selective TEAD Degrader with Durable Degradation ActivityAdvanced ScienceOctober 5, 2025Linhui Cao, Jing Yang, Yuhang Liu, Xiaotong Chen, Yufang Shi, Yunshuo Zhao, Yong Zhang, Jian Chen, Bowen Li, Wuqiang Wen, et al.hours. Afterward, a mixture of Nano‐Glo Live Cell Substrate and Nano‐Glo LCS Dilution Buffer (Promega, #N2011) was added to the 384‐well plates at 6 µL per well and incubated for 20 min at room temperature. Finally,

(17) Pancreatic cells are resistant to KRAS(Q61L) expression due to hyperactive ERK/MAPK signaling and apoptosis inductionCancer Research CommunicationsOctober 1, 2025Rachel A. Burge, Lucas Bialousow, Thomas McFall, Logan Bamonte, Grayson Johnson, Merissa Smith, Silvia G. Vaena, Susana Comte-Walters, Lauren E. Ball, Stefano Berto, et al.Nano-Glo Live Cell Reagent was added to each well according to the manufacturer’s protocol (Promega, # N2011). The plates were then placed on an orbital shaker for 1 minute at 30 rpm. After incubation, the plate

(18) Targeting prostaglandin E2 receptor 2 in Schwann cells inhibits inflammatory pain but not inflammationNature CommunicationsSeptember 25, 2025Romina Nassini, Lorenzo Landini, Matilde Marini, Martina Chieca, Daniel Souza Monteiro de Araújo, Marco Montini, Pasquale Pensieri, Vittorio Donato Abruzzese, Gaetano De Siena, Jin Zhang, et al.cells were treated for 10 min with Nano-Glo® Live Cell Reagent, according to manufacture instructions (#N2011, Nano-Glo® Live Cell Assay System Promega). Then, hSCs were stimulated with PGE2 (100 nM), CGRP (10

(19) Building a potent TREM2 agonistic, biparatopic, common light chain antibodyMAbsAugust 13, 2025Ana da Silva Almeida, Melissa L. Geddie, Anil Bhate, Chao Quan, Joseph W. Arndt, Yang Jiao, Joseph C. Santoro, Liron Noiman, Ramya Vijayakumar, Jorge Sanchez-Salazar, et al.indicated concentrations, and incubated for 2 h at 37°C, after which 25 μL of Nano-Glo Reagent (Promega #N2011) was added and luminescence was measured in a luminometer. THP1 P-SYK MSD assay 150,000 cells per

(20) PANCS-Binders: a rapid, high-throughput binder discovery platformNat MethodsAugust 6, 2025Matthew J. Styles, Joshua A. Pixley, Tongyao Wei, Christopher Basile, Shannon S. Lu, Bryan C. Dickinsonthe nano-luciferase activity was measured using Nano-Glo Live Cell Assay System (Promega, cat. no. N2011). Endogenous KRAS degradation assay Here, 1,000 ng of binder–LIR fusion plasmids were transfected

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Nano-Glo Live Cell Assay System

Nano-Glo Live Cell Assay System from Promega

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Nano-Glo Live Cell Assay System from Promega

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