Description
Principle of the assay: alphaMSH ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-alphaMSH antibody and an alphaMSH-HRP conjugate. The assay sample and buffer are incubated together with alphaMSH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the alphaMSH concentration since alphaMSH from samples and alphaMSH-HRP conjugate compete for the anti-alphaMSH antibody binding site. Since the number of sites is limited, as more sites are occupied by alphaMSH from the sample, fewer sites are left to bind alphaMSH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The alphaMSH concentration in each sample is interpolated from this standard curve