Description
Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PACAP. Standards or samples are added to the appropriate microtiter plate wells with biotin-conjugated PACAP. A competitive inhibition reaction is launched between PACAP (Standards or samples) and biotin-conjugated PACAP with the pre-coated antibody specific for PACAP. The more amount of PACAP in samples, the less antibody bound by biotin-conjugated PACAP. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of PACAP in the sample. The color development is stopped and the intensity of the color is measured.
Background: Pituitary adenylate cyclase-activating peptide, adenylate cyclase-activating polypeptide 1. Mediated by adenylate cyclase-activating polypeptide 1 receptors, this polypeptide stimulates adenylate cyclase and subsequently increases the cAMP level in target cells. Adenylate cyclase-activating polypeptide 1 not only is a hypophysiotropic hormone but also functions as a neurotransmitter and neuromodulator. In addition, it plays a role in paracrine and autocrine regulation of certain types of cells. This gene is composed of five exons. Exons 1 and 2 encode the 5' UTR and signal peptide, respectively; exon 4 encodes an adenylate cyclase-activating polypeptide 1-related peptide; and exon 5 encodes the mature peptide and 3' UTR