Description
Introduction: Interleukin-10 (IL-10), is an anti-inflammatory cytokine. This cytokine is produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappaB activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. A study in mice has shown that interleukin-10 is also produced by mast cells, counteracting the inflammatory effect that these cells have at the site of an allergic reaction.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the 3 appropriate microtiter plate wells with an antibody specific for IL-10 and Horseradish Peroxidase (HRP) conjugated IL-10, and incubated. A competitive inhibition reaction is launched between IL-10 (Standards or samples) and Horseradish Peroxidase (HRP) conjugated IL-10 with the antibody specific for IL-10. Then substrate solutions are added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IL-10 in the samples is then determined by comparing the O.D. of the samples to the standard curve