Description
Description: MitoPT TMRM assay kits utilize the well-characterized, lipophilic but slightly cationic fluorescent dye TMRM to detect mitochondrial membrane permeability. Use of the MitoPT TMRM kit allows for the easy distinction of apoptotic or mitochondrial membrane-compromised cells from non-apoptotic cells with polarized mitochondria. Cells with polarized mitochondria will fluoresce bright red (em max: 573 nm); cells with membrane- compromised cells will exhibit a dimished fluorescence or no signal. The MitoPT TMRM mitochondrial membrane permeability assay can be used in conjunction with existing apoptosis or metabolic stress protocols. Each kit includes the TMRM potentiometric dye reagent, a 10X wash buffer for removing any excess TMRM dye after staining, and the generic mitochondrial membrane depolarizer, carbonylcyanide m- chlorophenylhydrazone (CCCP). TMRM-stained cells can be analyzed using flow cytometry, fluorescence microscopy, and fluorescence plate reader evaluation methodology.
Background: Detect Mitochondrial Permeability Transition and Mitochondrial Membrane Depolarization with the MitoPT TMRM Assay ICT's MitoPT TMRM Assay utilizes the well-characterized potentiometric fluorescent dye tetramethylrhodamine methyl ester (TMRM) to detect mitochondrial permeability and membrane depolarization in whole, living cells. The rhodamine-based TMRE and TMRM dyes offered in ICT's MitoPT cell-based assay kits are categorized as slow membrane redistribution dyes. The lipophilic structures of hese dyes allow them to easily penetrate cell and mitochondrial lipid bilayer membrane barriers. Their potentiometric fluorescent properties allow for the detection of the mitochondrial permeability transition in whole cells via fluorescence analysis methods