Description
Principle of the assay: The CBI T3 is a solid phase competitive ELISA. The samples, T3 Antibody-Biotin Solution and the diluted T3 enzyme conjugate are added to the wells coated with Streptavidin. T3 in the patient's serum competes with a T3 enzyme (HRP) conjugate for binding sites. Unbound T3 and T3 enzyme conjugate is washed off by wash buffer during a wash step. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of T3 in the samples. A standard curve generated relating color intensity to the concentration of the T3