Description
Background/Introduction: Luteotropic hormone is a peptide hormone discovered by Dr. Henry Friesen, primarily associated with lactation. In breastfeeding, the act of an infant suckling the nipple stimulates the production of prolactin, which fills the breast with milk via a process called lactogenesis, in preparation for the next feed. Oxytocin, another hormone, is also released, which triggers milk let-down.Prolactin (PRL) is a peptide hormone primarily associated with lactation. In breastfeeding, the infant suckling the teat stimulates the production of prolactin, which fills the breast with milk (lactogenesis) in preparation for the next feed. Oxytocin, a similar hormone, is also released, which triggers milk let-down. Prolactin (PRL) is a polypeptide hormone secreted by anterior pituitary of both male and female.
Principle of the Assay: This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for LH and Horseradish Peroxidase (HRP) conjugated LH. The competitive inhibition reaction is launched between with HRP labeled LH and unlabeled LH with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of LH in the sample. The color development is stopped and the intensity of the color is measured