Description
Principle of the assay: The reduction potential of the sample or standard effectively converts Cu+2 to Cu+1, thus changing the ion's absorption characteristics. This reduced form of copper will selectively form a stable 2:1 complex with the chromogenic reagent with an absorption maximum at ca. 450 nm12-16. A known concentration of Trolox is used to create a calibration curve, with the data being expressed as mM Trolox equivalents or in muM copper reducing equivalents. A study was performed to evaluate the correlation between the concentration of principal antioxidants in human serum and the value obtained for total antioxidant power using this method with the results presented in Figure 1. Multivariate analysis of the obtained results yields a very high significance. The results obtained with the Total Antioxidant Power Assay for a series of serum samples were also compared to the resistance to oxidation of the serum lipids in these samples. The results of this study also show that these two parameters are highly correlated. The higher the total antioxidant power, the more protected the serum lipids are from oxidation.
Background: The reduction potential of the sample or standard effectively converts Cu+2 to Cu+1, thus changing the ion's absorption characteristics. This reduced form of copper will selectively form a stable 2:1 complex with the chromogenic reagent with an absorption maximum at ca. 450 nm12-16. A known concentration of Trolox is used to create a calibration curve, with the data being expressed as mM Trolox equivalents or in muM copper reducing equivalents. A study was performed to evaluate the correlation between the concentration of principal antioxidants in human serum and the value obtained for total antioxidant power using this method with the results presented in Figure 1. Multivariate analysis of the obtained results yields a very high significance. The results obtained with the Total Antioxidant Power Assay for a series of serum samples were also compared to the resistance to oxidation of the serum lipids in these samples. The results of this study also show that these two parameters are highly correlated. The higher the total antioxidant power, the more protected the serum lipids are from oxidation