Description
Intended Uses: Mouse tPA total antigen assay is intended for the quantitative determination of total tissue plasminogen activator in mouse plasma and other biological fluids.
Principle of the Assay: Mouse tPA will bind to the capture antibody coated on the microtiter plate. Free and complexed enzyme will react with the capture antibody on the plate. A standard calibration curve is prepared using dilutions of tPA along with the samples to be measured. After appropriate washing steps, monoclonal anti-mouse tPA primary antibody binds to the captured enzyme. Excess antibody is washed away and bound monoclonal antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. The amount of color development is directly proportional to the concentration of tPA antigen in the sample