Description
Principle of the assay: human P53 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for P53 has been precoated onto 96-well plates. Standards(NSO, M1-D393) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for P53 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human P53 amount of sample captured in plate.
Background: P53 (also known as protein 53 or tumor protein 53), is a tumor suppressor protein that in humans is encoded by the TP53 gene. The human P53 gene is mapped to chromosome 17. Human p53 is 393 amino acids long and has seven domains. It runs as a 53-kilodalton (kDa) protein on SDS-PAGE. The pattern of p53 splicing was specific for brain areas and for individuals. And human kidney and heart expressed only full-length p53. It has played a vital role in conserving stability by preventing genome mutation