Description
Introduction: Oxyntomodulin is a naturally-occurring 37-amino acid peptide hormone found in the colon, produced by the oxyntic (fundic) cells of the oxyntic (fundic) mucosa. It has been found to suppress appetite. A recent study has found that it can be used as a weight loss treatment. The mechanism of action of oxyntomodulin is not well understood. It is known to bind both the GLP-1 receptor and the glucagon receptor, but it is not known whether the effects of the hormone are mediated through these receptors or through an unidentified receptor.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to oxyntomodulin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for oxyntomodulin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain oxyntomodulin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of oxyntomodulin in the samples is then determined by comparing the O.D. of the samples to the standard curve