Description
Intended use The Chlamydia Trachomatis IgA ELISA kit is intended for the detection of IgA antibody to Chlamydia Trachomatis in human serum or plasma. For research use only, not for use in diagnostic procedures.
Principle of the test Diluted serum (serum diluent contains sorbent to remove rheumatoid factor and human IgG interference) is added to wells coated with purified antigen. IgA specific antibody, if present, binds to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibody-antigen complex, if present. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the hydrolysis of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of IgA specific antibody in the unknown