Description
For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
INTENDED USE This VD ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of mouse VD. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
PRINCIPLE OF THE ASSAY VD ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-VD antibody and an VD-HRP conjugate. The assay sample and buffer are incubated together with VD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the VD concentration since VD from samples and VD-HRP conjugate compete for the anti-VD antibody binding site. Since the number of sites is limited, as more sites are occupied by VD from the sample, fewer sites are left to bind VD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VD concentration in each sample is interpolated from this standard curve