Description
Intended Uses: This FGF7 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine FGF7. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay: FGF7 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FGF7 antibody and an FGF7-HRP conjugate. The assay sample and buffer are incubated together with FGF7-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FGF7 concentration since FGF7 from samples and FGF7-HRP conjugate compete for the anti-FGF7 antibody binding site. Since the number of sites is limited, as more sites are occupied by FGF7 from the sample, fewer sites are left to bind FGF7-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FGF7 concentration in each sample is interpolated from this standard curve