Description
Introduction: The Mouse tPA total antigen assay is intended for the quantitative determination of total plasminogen activator antigen in biological fluids. Tissue plasminogen activator (tPA) is a serine protease that converts plasminogen to the active serine protease plasmin in the blood fibrinolytic system1,
2. It also plays an important role in the removal of incipient thrombi
3. tPA is widely used for the thrombolytic treatment of acute myocardial infarction3.
Principle of assay: Mouse tPA will bind to the capture antibody coated on the microtiter plate. Free and complexed enzyme will react with the capture antibody on the plate. A standard calibration curve is prepared using dilutions of tPA along with the samples to be measured. After appropriate washing steps, monoclonal anti-mouse tPA primary antibody binds to the captured enzyme. Excess antibody is washed away and bound monoclonal antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. TMB substrate is used for color development at 450 nm