Description
For samples: Serum, plasma, cell culture superna tants, body fluid and tissue homogenate
INTENDED USE This TAb ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep TA b. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY TAb ELISA kit applies the competitive enzyme immunoassay technique utilizin gThyroid antigen and an TAb-HRP conjugate. The assay sample and buffer are incubated together with TAb-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TAb concentration since TAb from samples and TAb-HRP conjugate compete for the Thyroid antigen binding site. Since the number of sites is limited, as more sites are occupied by TAb from the sample, fewer sites are left to bind TAb-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TAb concentration in each sample is interpolated from this standard curve