Description
Principle of the assay: The MyBioSource Mouse/Rat T3 ELISA is a solid phase competitive ELISA. The samples, the working T3 enzyme conjugate, diluted in assay diluent, are added to the wells coated with anti-T3 polyoclonal antibody. T3 in the serum competes with T3 enzyme conjugate for binding sites. Unbound T3 and T3 enzyme conjugate is washed off by washing buffer. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of T3 in the samples. A standard curve is prepared relating color intensity to the concentration of the T3