Description
Principle of the assay: mouse IL-31 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-31 has been precoated onto 96-well plates. Standards(E.coli, T24-C163) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-31 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-31 amount of sample captured in plate.
Background: Interleukin-31, also known as IL-31 is a protein that in humans is encoded by the IL31 gene. The IL31 gene is mapped to chromosome 12q24.31 and the mouse gene maps to a syntenic region on chromosome 5. IL31, which is made principally by activated Th2-type T cells, interacts with a heterodimeric receptor consisting of IL31RA and OSMR that is constitutively expressed on epithelial cells and keratinocytes. IL31 may be involved in the promotion of allergic skin disorders and in regulating other allergic diseases, such as asthma