Description
Principle of the assay: The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to substance P. A competitive inhibition reaction is launched between biotin labeled substance P and unlabeled substance P (Standards or samples) with the pre-coated antibody specific to substance P. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of substance P in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the substance P level in the sample or standard