Description
Principle of the assay: The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to IAA. A competitive inhibition reaction is launched between biotin labeled IAA and unlabeled IAA (Standards or samples) with the pre-coated antibody specific to IAA. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of IAA in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the IAA level in the sample or standard