Maxwell RSC ccfDNA Plasma Kit from Promega

Product Specs
ItemMaxwell RSC ccfDNA Plasma Kit
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS1480
Quantity48 preps
SourceBodily Fluid, Plasma/Serum
Nucleic AcidCell Free DNA
Sample TypePlasma

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Adapting Clinical Chemistry Plasma as a Source for Liquid BiopsieseLifeMay 1, 2026Spencer C Ding, Jingru Yu, Tiepeng Liao, Lauren S Ahmann, Yvette Y Yao, Chandler Ho, Linlin Wang, Benjamin A Pinsky, Wei GumL of plasma using the Maxwell RSC 48 instrument (Promega) with the Maxwell RSC ccfDNA Plasma Kit (AS1480), following the manufacturer’s instructions. Samples were processed in parallel in batches. cfDNA concentrations

(2) Adapting Clinical Chemistry Plasma as a Source for Liquid BiopsiesmedRxivFebruary 27, 2026Spencer C. Ding, Jingru Yu, Tiepeng Liao, Lauren S. Ahmann, Yvette Y. Yao, Chandler Ho, Linlin Wang, Benjamin A. Pinsky, Wei GumL of plasma using the Maxwell RSC 48 instrument (Promega) with the Maxwell RSC ccfDNA Plasma Kit (AS1480), following the manufacturer’s instructions. Samples were processed in parallel in batches. cfDNA concentrations

(3) Sorafenib as first-line therapy for metastatic uveal melanoma: A multicenter, placebo-controlled randomized discontinuation study (STREAM)iScienceDecember 19, 2025Halime Kalkavan, Max E. Scheulen, Eckhart Kämpgen, Ulrich Keilholz, Lucie Heinzerling, Smiths S. Lueong, Annalena Hlinka, Tanja Gromke, Sebastian Ochsenreither, Ralf-Axel Hilger, et al.assays,Critical commercial assays ,, Maxwell RSC® ccfDNA plasma kit,"Promega Corporation, Madison, USA",Cat# AS1480 Droplet Digital PCR Assays for GNAQ p.Q209P c.626A>CGNAQ p.Q209L c.626A>TGNA11 p.Q209L c.626A>T,BIORAD,dHsaCP2506794dHsaCP2506795dHsaMDM5827925071dHsaMDW5827925073dHsaCP2000049dHsaMDW8418189543 ,, Software

(4) Plasma cell-free DNA methylation-based prognosis in metastatic castrate-resistant prostate cancernpj Precision OncologyDecember 13, 2025Jodie Wong, Yijun Tian, Manishkumar S. Patel, Kapil Avasthi, Claire Hanson, Matt Larsen, Enos Ampaw, Rebekah Gutowski, Muhammad Z. H. Fadlullah, Joseph Finkelstein, et al.of plasma was used for automated cfDNA extraction with the Maxwell RSC ccfDNA Plasma Kit (Promega, #AS1480). cfDNA quantification was performed using Qubit. Capture probe design 437 targeted methylation regions

(5) Prognostic value of circulating HPV cell-free DNA in cervical cancer using liquid biopsySci RepApril 3, 2025Ashna Gupta, Gunjan Dagar, Sumit Kr. Das, Ravi Chauhan, Abhishek Shankar, Daya Nand Sharma, Vaishali Suri, Maroof Ahmad Khan, Muzafar A. Macha, Ikhlak Ahmed, et al.cell-free DNA (cfDNA) was isolated from 1,000 µL of plasma using the Maxwell RSC® ccfDNA Plasma Kit (AS1480; Promega, Madison, WI, USA), a magnetic bead-based automated system. For each patient sample, 1 mL of

(6) Synergic activity of FGFR2 and MEK inhibitors in the treatment of FGFR2-amplified cancers of unknown primaryMolecular TherapyOctober 2, 2024Andrea Cavazzoni, Irene Salamon, Claudia Fumarola, Giulia Gallerani, Noemi Laprovitera, Francesco Gelsomino, Mattia Riefolo, Karim Rihawi, Elisa Porcellini, Tania Rossi, et al.5130450, Qiagen). Cell-free DNA was extracted from 1 mL plasma with Maxwell RSC ccfDNA Plasma Kit (Cat No: AS1480, Promega). Tumor DNA was extracted using the QIAamp DNA FFPE Tissue Kit (Cat No: 56404, Qiagen, Hilden,

(7) Circadian rhythm and circulating cell-free DNA release on healthy subjectsSci RepDecember 7, 2023Geoffroy Poulet, Jean-Sébastien Hulot, Anne Blanchard, Damien Bergerot, Wenjin Xiao, Frederic Ginot, Audrey Boutonnet-Rodat, Abdelli Justine, Guillaume Beinse, Vanna Geromel, et al.10 min at 4 °C. The DNA was extracted using the Maxwell RSC ccfDNA Plasma Kit (Promega, Cat No./ID: AS1480) following the manufacturer’s instruction and was eluted in 62µL of elution buffer. The DNA concentration

(8) BL-MOL-AR Project, Preliminary Results about Liquid Biopsy: Molecular Approach Experience and Research Activity in Oncological SettingsGlobal Medical GeneticsSeptember 1, 2023Alessandro Pancrazzi, Francesco Bloise, Alice Moncada, Roberta Perticucci, Stefania Vecchietti, Francesca Pompili, Francesca Ricciarini, Silvia Lenzi, Cristina Gatteschi, Sabrina Giusti, et al.performed with the Maxwell RSC Extractor, code AS4500, using the Maxwell RSC ccfDNA Plasma Kit, code AS1480 (Promega SRL, Milan, Italy). 22 The amount of extracted cfDNA was measured spectrophotometrically (NanoDrop

(9) Cell-free DNA as a potential biomarker of differentiation and toxicity in cardiac organoidseLifeJune 1, 2023Silver B, Gerrish K, Tokar E.(Promega, miRNA Tissue Kit AS1460; Tissue DNA Kit AS1610) and cfDNA from media (Promega, ccfDNA Plasma Kit AS1480) were extracted using the Promega Maxwell RSC instrument. RNA quality and purity were assessed using

(10) Digital PCR-based evaluation of nucleic acid extraction kit performance for the co-purification of cell-free DNA and RNAHuman GenomicsDecember 31, 2022Jill Deleu, Kathleen Schoofs, Anneleen Decock, Kimberly Verniers, Sofie Roelandt, Angie Denolf, Joke Verreth, Bram De Wilde, Tom Van Maerken, Katleen De Preter, et al.the MagNA Pure 24 instrument (MAP; Roche, 07290519001), (5) Maxwell ccfDNA LV Plasma Kit (Promega, AS1480) in combination with the Maxwell RSC Instrument (MAX; Promega, AS4500), and (6) miRNeasy Serum/Plasma

(11) Multi-omic brain and behavioral correlates of cell-free fetal DNA methylation in macaque maternal obesity modelsNature CommunicationsSeptember 21, 2022Benjamin I. Laufer, Yu Hasegawa, Zhichao Zhang, Casey E. Hogrefe, Laura A. Del Rosso, Lori Haapanen, Hyeyeon Hwang, Melissa D. Bauman, Judy Van de Water, Ameer Y. Taha, et al.library preparation The cffDNA was extracted from serum using a Maxwell RSC cffDNA Plasma Kit (Promega; AS1480) by the Primate Assay Laboratory Core at the California National Primate Research Center. The brain

(12) Clinical relevance and prognostic role of preoperative cell-free single-stranded DNA concentrations in colorectal cancer patientsKorean Journal of Clinical OncologyDecember 31, 2021Hyun Soo Song, Dong Hyun Kang, Hyunjung Kim, Tae Sung Ahn, Tae Wan Kim, Moo-Jun Baekusing a Maxwell RSC Instrument (AS4500; Promega, Madison, WI, USA) and a Maxwell RSC ccfDNA Plasma Kit (AS1480, Promega) according to the user manual. In the last elution step, 50 μL X TE buffer was used according

(13) Genetic Characterization of Cancer of Unknown Primary Using Liquid Biopsy ApproachesFrontiers in Cell and Developmental BiologyJune 10, 2021Noemi Laprovitera et al.(Cat No: AS4500, Promega, Madison, WI, United States) using the Maxwell RSC ccfDNA Plasma Kit (Cat No: AS1480, Promega). Genomic and ccfDNA were quantified using the Qubit 4.0 Fluorometer (Cat No: Q33238, Thermo Fisher Scientific

(14) Baseline Hedgehog Pathway Activation and Increase of Plasma Wnt1 Protein Are Associated with Resistance to Immune Checkpoint Inhibitors in Advanced Non-Small-Cell Lung CancerCancersMarch 5, 2021Mehlman C, Takam Kamga P, Costantini A, Julié C, Dumenil C, Dumoulin J, Ouaknine J, Giraud V, Chinet T, Emile JF, et al.Transcription and Quantitative Polymerase Chain Reaction RNA was extracted from tumor tissue using the AS1480 Maxwell RSC Simply RNA tissue kits (Promega, Madison, WI, USA) according to the manufacturer’s instructions.

(15) Small EVs-Associated DNA as Complementary Biomarker to Circulating Tumor DNA in Plasma of Metastatic Colorectal Cancer PatientsPharmaceuticals (Basel)February 6, 2021Silvia Galbiati et al.Maxwell RSC instrumentation (Promega, Madison, WI, USA) combined with the Maxwell® RSC ccfDNA Plasma Kit (AS1480) and eluted with 60 μL of Elution Buffer. For the experiment performed with DNAse treatment, 250 μL

(16) High Circulating Sonic Hedgehog Protein Is Associated With Poor Outcome in EGFR-Mutated Advanced NSCLC Treated With Tyrosine Kinase InhibitorsFrontiers in oncologyJanuary 1, 2021Takam Kamga P, Swalduz A, Costantini A, Julié C, Emile JF, Pérol M, Avrillon V, Ortiz-Cuaran S, de Saintigny P, Leprieur EG.were performed as previously described (21). Briefly, RNA was extracted from tumor tissue using the AS1480 Maxwell RSC Simply RNA tissue kits (Promega, USA) according to the manufacturer’s instructions. One

(17) Origin of circulating free DNA in patients with lung cancerPLOS ONEJuly 7, 2020Tomonori Abe, Chiho Nakashima, Akemi Sato, Yohei Harada, Eisaburo Sueoka, Shinya Kimura, Atsushi Kawaguchi, Naoko Sueoka-Araganeperformed. DNA was isolated from 1 ml plasma using the Maxwell RSC ccfDNA plasma cartridge® (Product no. AS1480; Promega, Mannheim, Germany) according to the manufacturer’s instructions. Each DNA sample extracted

(18) Sequential ctDNA whole-exome sequencing in advanced lung adenocarcinoma with initial durable tumor response on immune checkpoint inhibitor and late progressionJournal for ImmunoTherapy of CancerJune 24, 2020Etienne Giroux Leprieur et al.and immediately conserved at −80°C. DNA extraction ctDNA from plasma samples was extracted using AS1480 Maxwell RSC ccf DNA Plasma Kit (Promega, USA), according to manufacturer’s instructions. DNA from whole

(19) Multicenter Evaluation of Circulating Cell-Free DNA Extraction and Downstream Analyses for the Development of Standardized (Pre)analytical Work FlowsClinical ChemistryJanuary 1, 2020Rita Lampignano et al.(4 sites) (all QIAGEN), Maxwell RSC ccfDNA Plasma Kit AX1115 (3 sites), Maxwell RSC ccfDNA Plasma Kit AS1480 (Promega) (1 site), and Chemagic CNA 4k kit special (PerkinElmer) (1 site) following the manufacturer instructions

(20) Optimisation of robust singleplex and multiplex droplet digital PCR assays for high confidence mutation detection in circulating tumour DNAScientific ReportsDecember 1, 2019Vicky Rowlands, Andrzej J. Rutkowski, Elena Meuser, T. Hedley Carr, Elizabeth A. Harrington, J. Carl Barrettice. cfDNA was isolated from up to 2 ml plasma using a Maxwell® RSC instrument with ccfDNA Plasma Kit (AS1480) from Promega (Madison, Wisconsin, USA), KingFisher Flex System from Thermo Fisher Scientific (Waltham,

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