Description
• Detection sensitivity limit of approximately 0.6 mU/mL
• Quantitative assay for measuring glutathione reductase activity within plasma, erythrocytes, tissues, and cell lysates
• Sufficient reagents to perform up to 100 assays, including standard curve and unknown samples
MyBioSource's OxiSelect Glutathione Reductase Assay Kit is a Glutathione reductase activity is defined as 1 unit of enzyme reducing 1 umole oxidized glutathione (GSSG) per minute at pH 7.6 and 25 degree C. The kit employs a simple enzymatic recycling reaction for glutathione quantification where the reduction of a chromagen is correlated to glutathione reductase enzymatic activity. The kit has a detection sensitivity limit of approximately 0.6 mU/mL. Each kit provides sufficient reagents to perform up to 100 assays, including standard curve and unknown samples.
Introduction: Glutathione reductase is a homodimeric enzyme that is a member of the flavoprotein disulfide oxidoreductases. It has an indirect impact in the prevention of oxidative damage in cells by helping to maintain intracellular reduced glutathione (GSH). Thus, measuring the activity of the enzyme is an indicator of oxidative stress. It is a ubiquitous enzyme that catalyzes the NADPH dependent reduction reaction of oxidized glutathione (GSSG) to reduced glutathione (GSH). Oxidized glutathione is reduced through a multi-step reaction in which glutathione reductase is reduced by NADPH, which in turn reacts with a GSSG molecule. This creates a disulfide interchange reaction that creates two GSH molecules and restores glutathione reductase to its oxidized form. Regenerated GSH is available to detoxify hydrogen peroxide. Maintenance of GSH is vital in oxidation-reduction processes as well as detoxification of hydrogen peroxide and organic peroxides brought on by inflammation in cells.
MyBioSource's OxiSelect Glutathione Reductase Assay Kit is a quantitative assay for measuring glutathione reductase activity within plasma, erythrocytes, tissues, and cell lysates. Glutathione reductase activity is defined as 1 unit of enzyme reducing 1 umole oxidized glutathione (GSSG) per minute at pH 7.6 and 25 degree C. The kit employs a simple enzymatic recycling reaction for glutathione quantification where the reduction of a chromagen is correlated to glutathione reductase enzymatic activity. The kit has a detection sensitivity limit of approximately 0.6 mU/mL. Each kit provides sufficient reagents to perform up to 100 assays, including standard curve and unknown samples