Description
Principle of the assay: SNX1 ELISA kit applies the competitive enzyme immunoassay technique utilizing SNX1 antigen and an SNX1-Ab-HRP conjugate. The assay sample and buffer are incubated together with SNX1-Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SNX1 concentration since SNX1 from samples and SNX1 pre-coated on the plate compete for the SNX1 Ab-HRP binding site.Since the number of sites is limited, as more sites are occupied by SNX1 from the sample, fewer sites are left to bind SNX1 pre-coated on the plate.A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SNX1 concentration in each sample is interpolated from this standard curve