Description
Introduction: Lipid peroxidation is a well-established mechanism of cellular injury in both plants and animals, and is used as an indicator of oxidative stress in cells and tissues. Lipid peroxides are unstable and decompose to form a complex series of compounds including reactive carbonyl compounds. Polyunsaturated fatty acid peroxides generate malondialdehyde (MDA) and 4-hydroxyalkenals (HAE) upon decomposition. The measurement of MDA has been used as an indicator of lipid peroxidation (1). This method is designed to assay MDA.
Principle of assay: This assay is based on the reaction of a chromogenic reagent, N-methyl-2-phenylindole (R1), with MDA at 45 degree C. One molecule of MDA reacts with 2 molecules of Reagent R1 to yield a stable chromophore with maximal absorbance at 586 nm