Description
• Quantify 8-isoprostane in about 3 hours
• Suitable for use with urine, plasma, serum, or cell lysates
• 8-iso-PGF2alpha included as positive control
8-iso-Prostaglandin F2alpha (8-isoprostane) is a stable by-product of lipid peroxides generated during oxidative stress. Our OxiSelect 8-Isoprostane Assay Kit provides a convenient method for absolute quantitation in just a few hours. The 8-Isoprostane ELISA is suitable for a variety of sample types including urine, plasma, and cell lysates.
Introduction: Lipid peroxidation is a well-defined mechanism of cellular damage in animals and plants. Lipid peroxides are unstable indicators of oxidative stress in cells that decompose to form more complex and reactive compounds such as isoprostanes. The isoprostanes are a type of eicosanoids produced non-enzymatically through the oxygen radical induced peroxidation of tissue phospholipids and lipoproteins. Isoprostanes are prostaglandin-like compounds that appear in normal plasma and urine samples, but are elevated by oxidative stress in tissue, plasma, and urine.
8-iso-Prostaglandin F2alpha (also known as 8-epi-PGF2alpha, 8-isoprostane, or 15-isoprostane F2t), is an isoprostane that has been shown to be useful for the assessment of oxidative stress in vivo. It is produced in membrane phospholipids from non-cyclooxygenase and cyclooxygenase peroxidation pathways derived from arachidonic acid. 8-iso-Prostaglandin F2alpha (8-iso-PGF2alpha) is a potent vasoconstrictor, a mutagen in 3T3 cells as well as vascular smooth muscle cells, and also a possible pathophysiological mediator that can alter membrane integrity. It has been implicated in atherogenesis and elevated levels are associated with hepatorenal syndrome, rheumatoid arthritis, carcinogenesis, as well as atherosclerosis. 8-iso-PGF2alpha circulates in the plasma and is excreted in the urine. 8-iso PGF2alpha circulates as an esterified LDL Phospholipid and as a free acid. Normal human plasma and urine 8-iso PGF2alpha is about 40-100 pg/mL and about 190 pg/mg of creatinine respectively. Methods for determining total 8-iso PGF2alpha usually require alkaline hydrolysis of 8-iso PGF2alpha esters from tissues followed by extractions, phase separations and thin layer chromatography