Description
• Fully quantify chemotaxis with no manual cell counting
• Measure chemotaxis in less than 6 hours with most cell types
• Membrane inserts are uncoated to allow use with any chemoattractant
Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 8 um pore size is suitable for most cell types including epithelial cells, fibroblasts, and cancer cell lines.
Introduction: Cell migration is a highly integrated, multistep process that orchestrates embryonic morphogenesis, tissue repair and regeneration. It plays a pivotal role in the disease progression of cancer, mental retardation, atherosclerosis, and arthritis. The initial response of a cell to a migration-promoting agent is to polarize and extend protrusions in the direction of the attractant; these protrusions can consist of large, broad lamellipodia or spike-like filopodia. In either case, these protrusions are driven by actin polymerization and can be stabilized by extracellular matrix (ECM) adhesion or cell-cell interactions (via transmembrane receptors).
MyBioSource's CytoSelect Cell Migration Assay Kit utilizes polycarbonate membrane inserts (8 um pore size) to assay the migratory properties of cells. The kit contains sufficient reagents for the evaluation of 12 samples. The 8 um pore size is optimal for epithelial and fibroblast cell migration. However, in the case of leukocyte chemotaxis, a smaller pore size (3 um) is recommended