Description
• Fully quantify haptotaxis with no manual cell counting
• Measure haptotaxis in less than 6 hours with most cell types
• Membrane inserts are precoated on the bottom with Collagen
Cell haptotaxis describes cell migration toward or along a gradient of chemoattractants or adhesion sites in the extracellular matrix. The CytoSelect Cell Haptotaxis Assays are ideal for determining the migratory properties of cells. The 8 um membrane pore size is ideal for epithelial cells and fibroblasts. The membrane serves as a barrier to distinguish between migratory and non-migratory cells.
Introduction: Cell migration is a highly integrated, multistep process that orchestrates embryonic morphogenesis, tissue repair and regeneration. It plays a pivotal role in the disease progression of cancer, mental retardation, atherosclerosis, and arthritis. The initial response of a cell to a migration-promoting agent is to polarize and extend protrusions in the direction of the attractant; these protrusions can consist of large, broad lamellipodia or spike-like filopodia. In either case, these protrusions are driven by actin polymerization and can be stabilized by extracellular matrix (ECM) adhesion or cell-cell interactions (via transmembrane receptors).
MyBioSource's CytoSelect Cell Haptotaxis Assay Kit utilizes polycarbonate membrane inserts (8 um pore size) to assay the migratory properties of cells, the bottom side of the insert is coated with Fibronectin. The kit contains sufficient reagents for the evaluation of 12 samples. The 8 um pore size is optimal for epithelial and fibroblast cell migration. However, in the case of leukocyte chemotaxis, a smaller pore size (3 um) is recommended