Description
• Detect live and dead cells by microscopy, fluorescence, or flow cytometry
• Quantify live cells on a standard microplate reader or fluorometer
• Precoated plate included
Adhesion to the extraceullular matrix is essential for the survival and propagation of many adherent cells. Apoptosis resulting from the loss of adhesion to the ECM is known as anoikis. Anoikis is involved in the physiological processes of tissue renewal and cell homeostasis. Our CytoSelect Anoikis Assays allow you to quantify and monitor anoikis in cells using a precoated plate. Live cells can be viewed under a microscope and quantified on a plate reader by MTT (colorimetric) or Calcein AM (fluorometric); both reagents are included in the kit. Dead cells are detected with the red EthD-1 reagent, also included.
Introduction: Adhesion to the extracellular matrix (ECM) is essential for survival and propagation of many adherent cells. Apoptosis that results from the loss of cell adhesion to the ECM, or inappropriate adhesion is defined as "anoikis". Anoikis, from the Greek word for homelessness, is involved in the physiological processes of tissue renewal and cell homeostasis.
A common feature of carcinoma development and growth is the ability of transformed cells to survive under "anchorage independent" or "spheroid" growth conditions. This resistance to anoikis has been shown to be involved in the loss of cell homeostasis, cancer growth, and metastasis. The inhibition of cell adhesion, spreading, and growth on the ECM is an impediment to the cellular healing process, thus making it a possible therapeutic target. Preventing anoikis and enhancing cell adhesion and spreading is a major goal in the development of cell transplantation techniques, including the therapeutic use of progenitor cells. Further studies aimed at controlling the molecular mechanisms of anoikis resistance will serve to define effective therapies for the treatment of many human malignancies.
The CytoSelect 96-well Anoikis Assay Kit provides a colorimetric and fluorometric format to measure anchorage-independent growth and monitoring anoikis propelled cell death. The kit contains sufficient reagents for the evaluation of 96 samples in a Hydrogel coated 96-well plate. Live cells are detected with MTT or Calcein AM. Cell death is detected with the Ethidium Homodimer (EthD-1)