Human Chlamydia pneumoniae IgG EIA Kit DEIA1726 from Creative Diagnostics

Product Specs
ItemHuman Chlamydia pneumoniae IgG EIA Kit
CompanyCreative Diagnostics
Price Supplier Page View Company Product Page
Catalog NumberDEIA1726
ApplicationsSemi-Quantitative
ReactivityHuman
ValidationWithin-run reproducibility

Between-run reproducibility
Summary of the evaluation studies
Paired serum samples collected during the outbreak of C. pneumoniae epidemics in 1995 in Sweden were analyzed for the seroconversion. The rate of seroconversion detected by CD methods was compared to the respective rates by a competitor's EIA's. an in-house MIFA and an in-house complement fixation methods. Seroconversion of IgG and IgA values and/or positive IgM was interpreted as an acute C. pneumoniae infection by CD methods.

Originally, the samples were grouped as:
- positive pairs, meaning acute primary or reinfection (n = 106)
- negative pairs, meaning no infection or past infection (n = 134)
ComponentsNote:
- Reagents are stored between 2°C and 8°C.
- The expiration date is printed on each component label and on the package. Do not use reagents after the expiration date.
- Avoid unnecessary exposure to light. This is merely a precaution. The light sensitive reagents are the conjugate and the TMB-substrate solution, the latter one is packaged in non-transparent plastic vials for protection.
1. MICROSTRIPS 12 X 8 wells
Coated Microstrips.
2. SAMPLE DILUENT 100 mL
Phosphate buffered saline with additives, a blue coloring reagent, and 0.05% Bronidox as preservative.
3a. CALIBRATOR (EIU=130) 1.0 mL
Diluted human serum with 0.05% Bronidox as preservative and a red coloring reagent. Potential biohazardous material.
3b. BORDERLINE CONTROL 1.0 mL
Diluted human serum with intermediate amount of the antibodies, containing 0.05 % Bronidox as a preservative and a red coloring reagent. Potential biohazardous material.
3c. POSITIVE CONTROL 1.0 mL
Diluted human serum with high amount of the antibodies, containing 0.05 % Bronidox as a preservative and a red coloring reagent. Potential biohazardous material.
4. CONJUGATE 30 mL
Buffered salt solution with additives, a red coloring reagent, horseradish peroxidase conjugated antihuman IgG (sheep) with 0.1% N-Methylisothiazolone as preservative.
5. TMB-SUBSTRATE SOLUTION, ready to use 18 mL
Citrate buffered solution of 3,3',5,5'-Tetramethylbenzidine and hydrogen peroxide with additives and 0.01% Kathon CG as preservative.
6. STOPPING SOLUTION 25 mL
0.45 M H2SO4
7. WASHING SOLUTION 100 mL
Concentrated citrate buffered saline, with additives, and 0.05 % Bronidox as preservative.
INCUBATION COVERS 2 pcs
REAGENT BASINS 6 pcs
Sample Typeserum, plasma
Quantity96T

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Human Chlamydia pneumoniae IgG EIA Kit from Creative Diagnostics

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