Dengue NS1 Ag ELISA kit DEIA-CL019 from Creative Diagnostics

Product Specs
ItemDengue NS1 Ag ELISA kit
CompanyCreative Diagnostics
Price Supplier Page View Company Product Page
Catalog NumberDEIA-CL019
Quantity96T
ApplicationsELISA
ReactivityHuman
Components1. Anti-NS1 Antibody Coated Microwells - (Assay Plate) Microwells are coated with Anti-NS1 antibodies (12 x 8 wells). Ready for use. Unused microwells should be resealed immediately and stored in the presence of a desiccant. Stable at 2-8°C until expiry.
2. HRP Conjugated Anti-NS1 MAb - One bottle 15 mL (Orange). Horseradish peroxidase conjugated Anti-NS1 monoclonal antibody with preservative (0.1% ProclinTM). Ready for use. Stable at 2-8°C until expiry.
3. Wash Buffer Concentrate - One bottle, 60 mL of 20x concentrate of phosphate buffered saline (pH 7.2-7.6) with Tween 20 and preservative (0.1% ProclinTM). Crystallisation may occur at low temperatures. To correct, incubate at 37°C until clear. Mix well. Dilute one part wash buffer concentrate with 19 parts of distilled water. Diluted buffer may be stored for one week at 2-25°C.
4. Serum Diluent 3 - Two bottles, 22 mL (Brown). Ready for use. Tris buffered saline (pH 7.2-7.6) with preservatives (0.1% ProclinTM) and additives. Stable at 2-8°C until expiry.
5. Tetramethylbenzidine TMB-One bottle, 15 mL. Ready for use. A mixture of 3,3',5,5'-tetramethylbenzidine and hydrogen peroxide in a citric-acid citrate buffer (pH 3.5-3.8). Stable at 2- 8°C until expiry.
6. Positive Control - One Purple -capped vial, 400 µL recombinant antigen (contains 0.1% ProclinTM and 50 µg/mL gentamycin sulphate). Stable at 2-8°C until expiry.
7. Calibrator - One Orange -capped vial, 600 µL recombinant antigen (contains 0.1% ProclinTM and 50 µg/mL gentamycin sulphate). Stable at 2-8°C until expiry.
8. Negative Control - One White-capped vial, 400 µL human serum (contains 0.1% ProclinTM, 50 µg/mL gentamycin sulphate and 0.1% sodium azide). Stable at 2-8°C until expiry.
9. Stop Solution - One bottle, 15 mL. Ready to use. 1M Phosphoric acid. Stable at 2-25°C until expiry
ValidationThe reproducibility of the Dengue Early ELISA was established by testing 8 samples three times each on three kit batch numbers on three different days. Within-run, between day, between batch and total precision were estimated by analysis of variance (ANOVA Type II) and are presented in Table 2.
Note: Standard Deviation results have been rounded to two decimal places for tabulation purposes.
*Index value is calculated by dividing the sample absorbance by the cut-off value
Sample Typeserum

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Creative Diagnostics

Phone: 1-631-624-4882 (USA)
44-161-818-6441 (Europe)

Email: info@creative-diagnostics.com

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Website: https://www.creative-diagnostics.com/

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Dengue NS1 Ag ELISA kit from Creative Diagnostics

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Dengue NS1 Ag ELISA kit from Creative Diagnostics

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