Mouse Epidermal Growth Factor, Egf ELISA Kit CKERS-Egf-013M from Creative Diagnostics

Product Specs
ItemMouse Epidermal Growth Factor, Egf ELISA Kit
CompanyCreative Diagnostics
Price Supplier Page View Company Product Page
Catalog NumberCKERS-Egf-013M
Quantity15 plates, 45 plates
ApplicationsELISA
Gene NameEgf epidermal growth factor [ Mus musculus (house mouse) ]
Temperature##Capture Antibody: ## 720µg of antigen-affinity purified goat anti-EGF. Centrifuge vial prior toopening. Reconstitute in 1.0 ml sterile water for a concentration of 720µg/ml. Following reconstitution the Capture antibodies may be stored at 2-8°C for up to 6 months. For long term storage it is recommended to aliquot into working volumes and store at -70°C in a manual defrost freezer.
##Detection Antibody: ##36 µg of biotinylated antigen-affinity purified goat anti-EGF. Centrifuge vial prior to opening. Reconstitute in 1.0 ml sterile 1.0% BSA in PBS for a concentration of 36 µg/ml. Following reconstitution the Detection antibodies may be stored at 2-8°C for up to 6 months. For long term storage it is recommended to aliquot into working volumes and store at -70°C in a manual defrost freezer.
##Mouse EGF Standard: ##55 µg of recombinant EGF. Centrifuge vial prior to opening. Reconstitute in 0.5 ml sterile 1.0% BSA in PBS for a concentration of 110µg/ml. The Standard may be stored at 2-8°C for one month or aliquoted and stored at -70°C for up to three months in a manual defrost freezer.
##UltraAvidin-HRP Conjugate: ##40µl vial. Upon receipt, UltraAvidin-HRP conjugate should be stored at 2-8°C, DO NOT FREEZE.
##TMB Liquid Substrate: ##Aspirate and wash plate 4 times. Add 100µl of TMB HRPO Microwell Substrate Standard Kinetic One Component "Ready Use" to each well. Incubate at room temperature and monitorcolor development with an ELISA plate reader at 450 nm with wavelength correction set at 540 nm or 570 nm. The reaction may be stopped after 15-20 minutes by adding 100µl of 2 M sulfuric acid to each well
Domain/Region/TerminusChromosome Location: 3 G3; 3 58.5 cM
NCBI Full Gene Nameepidermal growth factor
ReactivityMouse

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Creative Diagnostics

Phone: 1-631-624-4882 (USA)
44-161-818-6441 (Europe)

Email: info@creative-diagnostics.com

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Website: https://www.creative-diagnostics.com/

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Mouse Epidermal Growth Factor, Egf ELISA Kit from Creative Diagnostics

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Mouse Epidermal Growth Factor, Egf ELISA Kit from Creative Diagnostics

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