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A Guide to Choosing the Right ELISA Kit
ELISA Kit
Human Connective Tissue Growth Factor, CTGF ELISA Kit
Human Connective Tissue Growth Factor, CTGF ELISA Kit from Creative Diagnostics
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Creative Diagnostics for
Human Connective Tissue Growth Factor, CTGF ELISA Kit
Description
Quantitative
Product Specs
Company Info
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Product Specs
Item
Human Connective Tissue Growth Factor, CTGF ELISA Kit
Company
Creative Diagnostics
Price
Supplier Page
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Catalog Number
CKERS-CTGF-007H
Applications
ELISA
Components
Capture Antibody: 100 µg of antigen-affinity purified goat anti-CTGF. Centrifuge vial prior to opening. Reconstitute in 1.0 ml sterile water for a concentration of 100 µg/ml. Following reconstitution the Capture antibodies may be stored at 2-8°C for up to 6 months. For long term storage it is recommended to aliquot into working volumes and store at -70°C in a manual defrost freezer. Avoid repeated freeze and thaw cycles.
Detection Antibody: 50 µg of biotinylated antigen-affinity purified goat anti-CTGF. Centrifuge vial prior to opening. Reconstitute in 0.5 ml sterile water for a concentration of 100 µg/ml. Following reconstitution the Detection antibodies may be stored at 2-8°C for up to 6 months. For long term storage it is recommended to aliquot into working volumes and store at -70°C in a manual defrost freezer. Avoid repeated freeze and thaw cycles.
Human CTGF Standard: 1 µg of recombinant CTGF. Centrifuge vial prior to opening. Reconstitute in 1.0 ml sterile water for a concentration of 1 µg/ml. The Standard may be stored at 2-8°C for one month or aliquoted and stored at -70°C for up to three months in a manual defrost freezer. Avoid repeated freeze and thaw cycles.
UltraAvidin-HRP Conjugate: 40 µl vial. Upon receipt, UltraAvidin-HRP conjugate should be stored at 2-8°C, DO NOT FREEZE.
TMB Liquid Substrate:
(TMB Substrate should be at ambient temperature prior to use)
Aspirate and wash plate 4 times. Add 100 µl of TMB HRPO Microwell Substrate Standard Kinetic One Component "Ready Use" to each well. Incubate at room temperature and monitor color development with an ELISA plate reader at 450 nm with wavelength correction set at 540 nm or 570nm. The reaction may be stopped after 15-20 minutes by adding 100 µl of 2 M sulfuric acid to each well
NCBI Full Gene Name
cellular communication network factor 2
NCBI Gene Aliases
IGFBP8, CTGF, HCS24, NOV2
Reactivity
Human
Temperature
Capture Antibody: 100µg of antigen-affinity purified goat anti-CTGF. Centrifuge vial prior to opening. Reconstitute in 1.0 ml sterile water for a concentration of 100µg/ml. Following reconstitution the Capture antibodies may be stored at 2-8°C for up to 6 months. For long term storage it is recommended to aliquot into working volumes and store at -70°C in a manual defrost freezer.
Detection Antibody: 50µg of biotinylated antigen-affinity purified goat anti-CTGF. Centrifuge vial prior to opening. Reconstitute in 0.5 ml sterile water for a concentration of 100µg/ml. Following reconstitution the Detection antibodies may be stored at 2-8°C for up to 6 months. For long term storage it is recommended to aliquot into working volumes and store at -70°C in a manual defrost freezer.
Human CTGF Standard: 1 µg of recombinant CTGF. Centrifuge vial prior to opening. Reconstitute in 1.0 ml sterile water for a concentration of 1 µg/ml. The Standard may be stored at 2-8°C for one month or aliquoted and stored at -70°C for up to three months in a manual defrost freezer.
UltraAvidin-HRP Conjugate: 40µl vial. Upon receipt, UltraAvidin-HRP conjugate should be stored at 2-8°C, DO NOT FREEZE.
TMB Liquid Substrate: Aspirate and wash plate 4 times. Add 100µl of TMB HRPO Microwell Substrate Standard Kinetic One Component "Ready Use" to each well. Incubate at room temperature and monitorcolor development with an ELISA plate reader at 450 nm with wavelength correction set at 540 nm or 570 nm. The reaction may be stopped after 15-20 minutes by adding 100µl of 2 M sulfuric acid to each well
Quantity
10 plates
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Creative Diagnostics
Phone
: 1-631-624-4882 (USA)
44-161-818-6441 (Europe)
Email: info@creative-diagnostics.com
Company Profile
Website:
https://www.creative-diagnostics.com/
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Human Connective Tissue Growth Factor, CTGF ELISA Kit from Creative Diagnostics
Supplier Page
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Product Specs
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